11。Neurosci Lett. 2006 Oct 9; [Epub ahead of print] Related Articles, Links
Expression analyses of 27 DNA repair genes in astrocytoma by TaqMan low-density array.
Zheng J, Jin H, Xingang L, Yuquan J, Wei Z, Daru L.
Laboratory of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China; Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China; State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
DNA repair systems act to maintain genome integrity in the face of replication errors, environmental insults, and the cumulative effects of age. The mRNA expressions of 27 genes of the DNA repair system as well as their correlation with the clinical characteristics were studied in human astrocytoma. We applied TaqMan low-density array to investigate the mRNA expressions of 27 DNA repair genes in 40 astrocytoma tissues (10 of grade II, 10 of grade III, and 20 of grade IV, according to the WHO Grading System). And the normal brain tissues from 10 non-astrocytoma patients were collected as the control. In addition, correlation of their mRNA levels with clinical characteristics was also analyzed. We found that the expression of the 13 genes were significantly (P<0.01) down-regulated in grade II, III, IV of astrocytoma compared to normal brain tissues, including ERCC1, ERCC2, ERCC3, ERCC4, MGMT, MLH1, MLH3, NTHL1, OGG1, RAD50, SMUG1, XRCC4 and XRCC5. Meanwhile, we found that the expression of MSH2, MSH6, NUDT1 and XRCC3 were only significantly lower in grade II and III of astrocytoma, and the expression of MRE11A and MUS81 were only significantly lower in grade III and IV. But the expression of MPG, MSH3, MUTHY and RAD51 were not changed in any grade of astrocytoma. Furthermore, we found that the decrease expression of eight genes was significantly (P<0.05) associated with a poor prognosis, including ERCC3, ERCC4, MLH3, MRE11A, NTHL1, RAD50, XRCC4 and XRCC5. We suggest that TaqMan low-density array is an effective multivariate technique to examine the expression of DNA repair genes in astrocytomas, which can be applied to identify tumor-specific genes. We also suggest that the down-regulation of some DNA repair genes may be associated with pathogenesis and poor prognosis of astrocytoma.
齐鲁神外李新刚教授。
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0G-4M340G8-3&_user=1730128&_handle=V-WA-A-W-CD-MsSWYWW-UUA-U-AAZBVDWWYC-AAZAUCBUYC-WUUYCCEVU-CD-U&_fmt=full&_coverDate=10%2F10%2F2006&_rdoc=11&_orig=browse&_srch=%23toc%234862%239999%23999999999%2399999!&_cdi=4862&view=c&_acct=C000054345&_version=1&_urlVersion=0&_userid=1730128&md5=c3c9827f6e760053bee62d43eccf3aa12. Immunogenetics. 2006 Oct 11; [Epub ahead of print] Related Articles, Links
Association of interferon-gamma gene haplotype in the Chinese population with hepatitis B virus infection.
Liu M, Cao B, Zhang H, Dai Y, Liu X, Xu C.
Medical College, Shandong University, Jinan, Shandong Province, China.
In general, cytokines encoded by different genes of human genome might strongly influence host cell-mediated immune responses, which play an important role in the clearance of virus by the infected host. Interferon gamma (IFN-gamma) produced by T lymphocytes and natural killer cells plays an essential role in affecting cellular immune responses. A functional study demonstrated that two single nucleotide polymorphisms located in the IFN-gamma gene intron (at positions +874 and +2109) were involved in its transcriptional regulation. The aim of this study was to evaluate whether IFN-gamma gene polymorphisms or its haplotypes might be associated with predisposition to hepatitis B virus (HBV) infection in the Chinese population. The study included 181 cases with HBV infection and 272 gender, age-matched healthy controls. All genotyping were identified by polymerase chain reaction in association with the measurement of amplification refractory mutation system. A significant difference was observed between case and control groups. The frequency of +874A allele was significantly higher in patients than in controls (OR = 2.25, 95%CI = 1.69-2.99, P < 0.0001). However, no significant difference was found in the allelic frequencies of IFN-gamma +2109A/G between cases and controls (P > 0.05). By haplotype analysis, the frequency of haplotype AG (+874A and +2109G) revealed a significant difference in the cases in comparison to controls (P < 0.0001). Multiple logistic regression analysis showed that individuals possessing haplotype AG had an increased likelihood of HBV infection (OR = 8.14, 95%CI = 4.98-13.30). Our results suggest that haplotype AG containing +874A and +2109G may be a crucial risk factor of genetic susceptibility to HBV infection in the Chinese population.
PMID: 17033822 [PubMed - as supplied by publisher]
还没有全文，不知道是谁。可能是生化的刘贤锡教授。
3. Acta Biochim Biophys Sin (Shanghai). 2006 Oct;38(10):731-6. Related Articles, Links
Establishment and Application of a TaqMan Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Rubella Virus RNA.
Zhao LH, Ma YY, Wang H, Zhao SP, Zhao WM, Li H, Wang LY.
Department of Microbiology, School of Medicine, Shandong University, Jinan 250012, China.
zhaolihong7097@yahoo.com.cn.
The aim of this study was to establish and apply a real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) for rubella virus (RV) RNA. First, the primer and TaqMan probe concentrations, as well as reaction temperatures were optimized to establish an efficient real-time quantitative RT-PCR assay for RV RNA. Next, an RV-specific PCR amplicon was made as an external standard to estimate the linearity, amplification efficiency, analytical sensitivity and reproducibility of the real time quantitative assay. Finally, the assay was applied to quantify RV RNA in clinical samples for rubella diagnosis. The RV-specific PCR amplicon was prepared for evaluation of the assay at 503 bp, and its original concentration was 2.75x109 copies/mul. The real time quantitative assay was shown to have good linearity (R2=0.9920), high amplification efficiency (E=1.91), high sensitivity (275 copies/ml), and high reproducibility (variation coefficient range, from 1.25% to 3.58%). Compared with the gold standard, the specificity and sensitivity of the assay in clinical samples was 96.4% and 86.4%, respectively. Therefore, the established quantitative RT-PCR method is a simple, rapid, less-labored, quantitative, highly specific and sensitive assay for RV RNA.
PMID: 17033720 [PubMed - in process]
微生物教研室赵蔚明组。
4.J Interv Cardiol. 2006 Oct;19(5):367-75. Related Articles, Links
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Effect of Postconditioning on Coronary Blood Flow Velocity and Endothelial Function and LV Recovery After Myocardial Infarction.
Ma X, Zhang X, Li C, Luo M.
From the Department of Cardiology, Shandong Provincial Hospital of Shandong University, Jinan, China.
Objective: Postconditioning is a novel approach to myocardial protection during ischemia reperfusion. Our study observed the effect of postconditioning on coronary blood flow velocity and endothelial function in patients who underwent emergency percutaneous coronary intervention (PCI). Methods: Ninety-four patients with their first acute myocardial infarction who underwent revascularization within 12 hours of onset by primary PCI were recruited in the study. All the patients were randomized to two groups, IR group (PCI without postconditioning) and Postcond group (PCI with postconditioning). Corrected TIMI frame count (CTFC) was used to evaluate velocity of coronary blood after PCI. Creatine phosphokinase (CK), CK-MB, and malondialdehyde (MDA) were measured before and after PCI. Arterial endothelial function was studied noninvasively by examination of brachial artery responses to endothelium-dependent and endothelium-independent stimuli by echo Doppler technique. Wall motion score index (WMSI) was assessed by two-dimensional echocardiography before and 8 weeks after angioplasty. Results: There were no significant differences between the two groups with regard to age, sex, presence of angiographically visible collaterals, and elapsed time from the onset of symptoms until perfusion. Patients with postconditioning had much faster CTFC than patients without postconditioning (25.38 +/- 5.35 vs 29.23 +/- 5.54). After 8 weeks, the WMSI improved significantly in both groups, but the DeltaWMSI in Postcond group was significantly larger than that of IR group (1.20 +/- 0.30 vs 1.04 +/- 0.36, P < 0.05). There was a significant negative correlation between DeltaWMSI and CTFC in IR group and Postcond group (r =-0.9032, P < 0.01; r =-0.7884, P < 0.01). The peaks of CK and CK-MB of Postcond group were much lower than that of IR group (1236.57 +/- 813.21 U/L vs 1697.36 +/- 965.74 U/L; 116.92 +/- 75.83 U/L vs 172.41 +/- 92.64 U/L), and MDA-reactive products were significantly lower than that in the IR group at any same time after PCI. All patients with acute myocardial infarction had a depressed endothelium-dependent vasodilation function, while the endothelium-dependent vasodilation function was improved in Postcond group. Conclusion: Postconditioning is a simple, operative procedure for salvaging the coronary endothelial function and cardiomyocyte. It could be used widely in clinic and to better the prognosis of acute myocardial infarction.
PMID: 17020559 [PubMed - in process]
省立心内张兴华主任。
5. Gynecol Oncol. 2006 Sep 29; [Epub ahead of print] Related Articles, Links
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Voltage-gated K(+) channels are associated with cell proliferation and cell cycle of ovarian cancer cell.
Zhanping W, Xiaoyu P, Na C, Shenglan W, Bo W.
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, PR China.
OBJECTIVES: To study the role of Voltage-gated potassium ion channels (Kv) in cell proliferation and cell cycle of ovarian cancer cell. METHODS: The effects of voltage-gate K(+) channels on human ovarian cancer cell line (A2780 cell) proliferation and cell cycle were observed by means of MTT and flow cytometry. A variety of K(+) channel blockers were used in order to differentiate the critical subtype of K(+) channels involved. Mechanism of K(+) channel in cell proliferation was explored by studying the relationship between the K(+) channel and Ca(2+) entry. Kv and L-type Ca(2+) channel gene expressions were determined by RT-PCR. RESULTS: 4-Aminopyridine, an inhibitor of voltage-gated K(+) channels, significantly inhibited the proliferation of A2780 cells as measured by MTT. 4-Aminopyridine also significantly affected the cell cycle, which increased the percentage of cells in G0/G1, and reduced the percentage of cells in S phase and G2/M phase. Non-selective K(+) channel blockers tetrapentylammonium (TPeA) and verapamil had similar inhibitory effects on A2780 cell proliferation and cell cycle. In contrast, iberiotoxin, a selective inhibitor of K(Ca) channels, and glibenclamide, a potent inhibitor of K(ATP) channels, had no effect on the cell proliferation and cell cycle of A2780. Moreover K(+) channels inhibitor, TPeA and verapamil, can blocked Ca(2+) influx in these cells. CONCLUSION: Voltage-gated K(+) channels play an important role in the proliferation and cell cycle of ovarian cancer cell. It is likely that the influence of Kv channels on A2780 cell proliferation and cell cycle is mediated by a Ca(2+)-dependent mechanism.
PMID: 17014896 [PubMed - as supplied by publisher]
齐鲁妇产王波教授
6.Scand Cardiovasc J. 2006 Oct;40(5):312-7. Related Articles, Links
Limb ischemic postconditioning protects myocardium from ischemia-reperfusion injury.
Li CM, Zhang XH, Ma XJ, Luo M.
Department of Cardiology, Shandong Provincial Hospital of Shandong University, Jinan, 250021, China.
Objective. To test the hypothesis that limb ischemic postconditioning protects the myocardium from reperfusion injury, and examine the mechanism involved. Design. Forty rabbits were randomly divided into four groups: Control, Ischemic Preconditioning, Ischemic Postconditioning and Remote Postconditioning. Myocardial infarct size and tissue myeloperoxidase activity were determined at the end of the experiment. Plasma creatine kinase and malondialdehyde activity were measured at baseline, the end of ischemia, and after 3 h of reperfusion respectively. Results. Myocardial infarct size was significantly reduced in Ischemic Preconditioning, Ischemic Postconditioning and Remote Postconditioning as compared to Control (p < 0.01). Results were confirmed by plasma creatine kinase activity. Plasma malondialdehyde was significantly less at 3 h of reperfusion in Ischemic Preconditioning, Ischemic Postconditioning and Remote Postconditioning than that in Control (p < 0.01). Neutrophil accumulation (myeloperoxidase activity) in the area at risk was less in Ischemic Preconditioning, Ischemic Postconditioning and Remote Postconditioning than that in Control (p < 0.01). Conclusion. Remote postconditioning reduces myocardial infarction in rabbits. The mechanism involved might be reduced oxygen radical-induced injury and improved antioxidant action.
PMID: 17012143 [PubMed - in process]
省立心内张兴华主任
7.Brain Dev. 2006 Sep 26; [Epub ahead of print] Related Articles, Links
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Consequences of pilocarpine-induced recurrent seizures in neonatal rats.
Xiu-Yu S, Ruo-Peng S, Ji-Wen W.
Department of Pediatrics, Qilu Hospital, Shandong University, No. 107, Wen Hua Xi Road, Jinan, Shandong 250012, China.
Accumulated evidence have shown that a series of morphological alternations occur in patients with epilepsy and in different epileptic animal models. Given most of animal model studies have been focused on adulthood stage, the effect of recurrent seizures to immature brain in neonatal period has not been well established. This study was designed to observe the certain morphological changes following recurrent seizures occurred in the neonatal rats. For seizure induction, neonatal Wistar rats were intraperitoneally injected with pilocarpine on postnatal day 1 (P1), P4 and P7. Rat pups were grouped and sacrificed at 1d, 7d, 14d and 42d after the last pilocarpine injection respectively. Bromodeoxyuridine (BrdU) was intraperitoneally administered 36h before the rats were sacrificed. BrdU single and double labeling with neuronal markers were used to analyze cell proliferation and differentiation. Nissl and Timm staining were performed to evaluate cell loss and mossy fiber sprouting. Rats with neonatal seizures had a significant reduction in the number of Bromodeoxyuridine-(BrdU) labeled cells in the dentate gyrus compared with the control groups when the animals were killed either 1 or 7 days after the third seizure (P<0.05) but there was no difference between two groups on P21. On the contrary, BrdU-labeled cells significantly increased in the experimental group compared with control group on P49 (P<0.05). The majority of the BrdU-labeled cells colocalized with neuronal marker-NF200 (Neurofilament-200). Nissl staining showed that there was no obvious neuronal loss after seizure induction over all different time points. Rats with the survival time of 42 days after neonatal seizures developed to increased mossy fiber sprouting in both the CA3 region and supragranular zone of the dentate gyrus compared with the control groups (P<0.05). Taken together, the present findings suggest that synaptic reorganization only occurs at the later time point following recurrent seizures in neonatal rats, and neonatal recurrent seizures can modulate neurogenesis oppositely over different time window with a down-regulation at early time and up-regulation afterwards.
PMID: 17008043 [PubMed - as supplied by publisher]
齐鲁医院儿内科石秀玉
8.Med Hypotheses. 2006 Sep 26; [Epub ahead of print] Related Articles, Links
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Neurotrophic receptor TrkB: Is it a predictor of poor prognosis for carcinoma patients?
Han L, Zhang Z, Qin W, Sun W.
Institute of Immunology, Medical College, Shandong University, Jinan 250012, People's Republic of China.
The neurotrophic receptor tyrosine kinase TrkB, while binding its high affinity ligand brain-derived neurotrophic factor (BDNF), will play an essential role for nervous system development, neuronal survival, differentiation, and maintenance. However, accumulating evidences implies that TrkB signal pathway may also be involved in a variety of human cancers, in which TrkB is likely to play a role in initiation and metastasis of carcinomas. Overexpression of TrkB is often correlated with the tumorigenesis, angiogenesis and drug resistance in these malignancies, contributing significantly to the metastasis and aggressive phenotype of these poor prognosis tumors. The evidences to show the significant contribution of TrkB to malignancy not only came from solid tumors such as neoblastoma, pancreas cancer, Wilm's tumor and hepatocarcinoma, but also came from haematological malignancies such as Hodgkin lymphoma and multiple myeloma. In summary, besides its role in development and function of nervous system, TrkB is likely to also play a role in initiation and metastasis of carcinoma although it still remains to be further investigated and confirmed. Emerging data have suggested that TrkB may be a mediator as well as a marker of carcinogenesis and metastasis, therefore TrkB may be used as a valuable target for cancer therapy especially for the metastatic tumors with poor prognosis.
PMID: 17008023 [PubMed - as supplied by publisher]
免疫学教研室孙汶生教授
9. Arch Virol. 2006 Sep 28; [Epub ahead of print] Related Articles, Links
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Armored RNA as positive control and standard for quantitative reverse transcription-polymerase chain reaction assay for rubella virus.
Zhao L, Ma Y, Zhao S, Yang N.
Department of Microbiology, School of Medicine, Shandong University, Jinan, China.
RV Armored RNA constructed in the laboratory was shown to be resistant to RNase and DNase digestion. It could remain stable at 4 degrees C for at least 60 days, and was much more stable than RV strain JR23 in normal human plasma. Therefore it could be used as stable, well-characterized positive control and standard for quantitative RT-PCR assay.
PMID: 17006599 [PubMed - as supplied by publisher]
同前，微生物教研室赵丽红（音译）
10. Oral Oncol. 2006 Sep 22; [Epub ahead of print] Related Articles, Links
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Combined effects of soluble vascular endothelial growth factor receptor FLT-1 gene therapy and cisplatin chemotherapy in human tongue carcinoma xenografts.
Gao ZN, Wei YQ, Yang PS, Xu X, Zhao HQ, Huan X, Kang B.
State Key Laboratory of Biotherapy, Sichuan University, Chengdu City 610041, PR China; College of Stomatology, Shandong University, Number 44, Wen Hua Xi Lu, Jinan City, Shandong Province 250012, PR China.
The aim of the present study was to assess the anti-tumor effect of a defective adenovirus that expresses soluble vascular endothelial growth factor (VEGF) receptor FLT-1 (AdsFLT-1) in combination with cisplatin (cis-diamminedichloroplatinum, DDP) on human tongue carcinoma Tca8113 cell xenografts that had been pre-established in nude mice. In vitro, Tca8113 cells secreted soluble FLT-1 (sFLT-1) after infection with AdsFLT-1, and the conditioned medium from AdsFLT-1-treated Tca8113 cells seemed to inhibit VEGF-induced proliferation of human umbilical vein endothelial cells. The combined effects of sFLT-1 gene therapy and DDP chemotherapy was then studied in well-established Tca8113 xenografts. The concentration of sFLT-1 in serum reached a peak 8 days after intratumoral injection of AdsFLT-1. In these tumors, AdsFLT-1 intratumoral injections had only a small effect. Interestingly, when the cells were also exposed to DDP chemotherapy, significantly higher (P<0.05), and possibly synergistic, anti-tumoral effects were observed that were highly correlated to a marked reduction in intratumoral vascularization and an increase in tumor-cell apoptosis. Together, these data emphasize the potential of combining an anti-angiogenic gene therapy strategy with a destructive approach directed against the tumor cells to fight human tongue carcinoma.
PMID: 16997614 [PubMed - as supplied by publisher]
口腔医学院高振南博士
11. Immunol Lett. 2006 Sep 15;107(1):71-5. Epub 2006 Aug 23. Related Articles, Links
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Construction of a sIgA-enhancing anti-Porphyromonas gingivalis FimA vaccine and nasal immunization in mice.
Guo H, Wang X, Jiang G, Yang P.
Stomatology Hospital of Shandong University, Jinan 250012, China.
Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Fimbriae are one of several critical surface virulence factors of P. gingivalis. Interleukin 15 (IL-15) is a critical important cytokine for the differentiation of B-1 cells into IgA-inducing cells in mucosal tissues and the proliferation of B cells. The present study constructed a co-expression plasmid pIRES-fimA:IL-15 encoding fimbrinllin (FimA), a subunit of fimbriae and IL-15 as a sIgA-enhancing anti-P. gingivalis FimA vaccine. The plasmid pIRES-fimA:IL-15 was transfected to CHO cells. The expressions of FimA and IL-15 in CHO cells were verified by Western blot and ELISA. Mice were immunized with pIRES-fimA:IL-15 via nasal or intramucusal route. The results showed that nasal immunization was capable of promoting Ag-specific immune responses in the oral region as well as systemic immunity. When immunized via nasal route, IL-15 expressed by the plasmid enhanced FimA-specific sIgA antibody response. In conclusion, a co-expression plasmid pIRES-fimA:IL-15 has been constructed, and when immunized via nasal route, antigen-specific sIgA antibody response could be modulated positively in immunized mice.
PMID: 16959327 [PubMed - in process]
口腔医院杨丕山教授
12. Toxicology. 2006 Oct 3;227(1-2):36-44. Epub 2006 Jul 10. Related Articles, Links
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Malondialdehyde and catalase as the serum biomarkers of allyl chloride-induced toxic neuropathy.
Wang QS, Zhang CL, Zhao XL, Yu SF, Xie KQ.
Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
Chronic exposure to allyl chloride (AC) is known to produce a central-peripheral distal axonopathy. To access the biomarker of exposure and elucidate the mechanism of neuropathy induced by AC, we performed a longitudinal observational study of malondialdehyde (MDA), anti-reactive oxygen species (anti-ROS), glutathione (GSH), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in rats serum and sciatic nerve after 0, 3, 6, 9 and 12 weeks of AC administration. AC was administrated to Wistar rats by gavage at a single dosage of 200mg/kg/per dose (three times per week). Rats were sacrificed after 0, 3, 6, 9 and 12 weeks of AC treatment, serum and sciatic nerves were quickly collected at 4 degrees C. The results showed that MDA levels in serum (115.4 and 126.2%) and sciatic nerve (130.5 and 145.3%) significantly increased (p<0.05) on 3rd week of AC treatment and at gait score of 2, and further changes of MDA levels were observed after 6, 9 and 12 weeks and at gait score of 3 and 4. While a decrease (p<0.05) in the activities of CAT on 6th week of AC intoxication and at gait score of 2 was observed in serum (81.2 and 72.8%) and sciatic nerve (71.7 and 70.7%). The other antioxidants also decreased in serum and sciatic nerve after 3, 6 and 9, 12 weeks' intoxication and at gait score of 2, 3 and 4. Significant (p<0.05) positive correlations were observed between serum and sciatic nerve in MDA levels (r=0.9162 and 0.9551, respectively) and CAT (r=0.9410 and 0.9557, respectively) activities as time went on and symptoms developed. Thus, AC intoxication was associated with elevation of lipid peroxidation and reduction of antioxidative status, and the time dependent changes of these indexes in Wistar rats' serum and sciatic nerve occurred. The misbalance of lipid peroxidation and antioxidation status might be one of mechanisms of toxic neuropathy induced by AC. MDA and CAT could be served as the biomarkers of AC exposure to afford the early diagnosis of AC-induced toxic neuropathy.
PMID: 16938375 [PubMed - in process]
公卫谢克勤教授
13. Biochem Biophys Res Commun. 2006 Sep 22;348(2):413-20. Epub 2006 Jul 26. Related Articles, Links
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Cell death induced by MPPa-PDT in prostate carcinoma in vitro and in vivo.
Tian Y, Leung W, Yue K, Mak N.
Department of Biochemistry, School of Medicine, Shandong University, Jinan, China.
Lack of effective photosensitizers has become a major limit for extensive application of photodynamic therapy. In this study, the photocytotoxicity and mode of death induced by a newly developed photosensitizer MPPa, a derivative of chlorophyll a, were investigated in PC-3M cell line, a highly metastatic variant of poorly differentiated androgen-independent proctanec adenocarcinoma PC-3. MTT reduction assay was used to measure cytotoxicity in both PC-3M and HUVEC, after which a flow cytometer was used to measure apoptotic rate and cell cycle, and then Caspase-3, -8, -9 were investigated. Finally, an animal model was set up to embody the curative effect and for histopathological examinations. The photocytotoxicity of MPPa showed both light- and drug-dose dependent characteristics and no significant dark cytotoxicity was observed in PC-3M cells. In HUVEC, MPPa exhibited an obviously low cytotoxicity. By other in vitro studies, we found MPPa-PDT induced apoptotic mainly via the mitochondrial/Caspase-9/Caspase-3 pathway and could restrain the cell cycle progression from the more sensitive G0/G1-phases. In vivo, the tumour growth was significantly inhibited after PDT, and many apoptotic cells could be seen by histopathological examinations. These results indicate the death way of cells induced by MPPa is mainly via mild apoptotic and the cure effect is obvious, suggesting that MPPa is a potential photosensitizer of photodynamic therapy for prostate cancer.
PMID: 16889752 [PubMed - indexed for MEDLINE]
生化教研室田媛媛
14. J Pharm Biomed Anal. 2006 Sep 18;42(2):218-22. Epub 2006 Jul 24. Related Articles, Links
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Study on warfarin plasma concentration and its correlation with international normalized ratio.
Sun S, Wang M, Su L, Li J, Li H, Gu D.
Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China.
A sensitive high-performance liquid chromatographic (HPLC) method was developed for warfarin determination in plasma of patients who undertook cardiac valve replacement and were on anticoagulation with warfarin. The method described proved to be accurate, sensitive, easy to perform, reproducible and specific for plasma warfarin measurement with relative standard deviation (R.S.D.) of <5.27% for inter-day and <6.89% for intra-day. The assay was linear in warfarin concentration ranges of 0.12-3mug/ml (r=0.9995) with mean recovery of 94.6%. The mean warfarin plasma concentration of 58 patients with heart valve replacement within 1 month of post operation was 567.6+/-122.3ng/ml. The anticoagulant effect of the drug was monitored by international normalized ratio (INR). The correlation of warfarin dosage and concentration with INR was analysed, and the coefficients were 0.21, 0.1<p<0.2 and 0.30, 0.02<p<0.1, respectively. The correlation of warfarin dosage or concentration with INR is very poor, and hence in order to adjust the dosage more objectively and accurately, concentration monitoring is necessary and helpful for the patient management. It is needed especially when the ideal INR is difficult to target.
PMID: 16860509 [PubMed - in process]
千佛山医院药剂科孙淑娟
15.J Pharm Biomed Anal. 2006 Sep 26;42(3):372-8. Epub 2006 Jun 9. Related Articles, Links
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Development and validation of a sensitive LC-MS/MS method for the determination of adefovir in human serum and urine.
Sun D, Wang H, Wang B, Guo R.
Department of Pharmacy, the Second Hospital of Shandong University, Jinan 250033, China.
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of adefovir (PMEA) in human serum and urine. The analyte was separated on a Diamonsil C(18) column (250mmx4.6mm i.d., 5mum particle size) by isocratic elution with methanol-water-formic acid (20:80:0.1, v/v/v) at a flow rate of 0.6ml/min, and analyzed by mass spectrometry in multiple reaction-monitoring mode. The precursor-to-product ion transitions of m/z 274-->162 and m/z 226-->135 were used to measure and quantify the analyte and internal standard (I.S.), respectively. The weighted (1/x(2)) calibration curve was linear over serum concentration range 1.25-160.00ng/ml and urine concentration range 0.05-8.00mug/ml, with a correlation coefficient (r) of 0.9992 and 0.9978, respectively. The lower limit of quantification in human serum was 1.25ng/ml. The inter- and intra-day precisions (R.S.D.%) in both serum and urine were lower than 8.64%, the mean method accuracies and recoveries from spiked serum samples at three concentrations ranged from 96.3 to 102.0% and 56.5 to 59.3%, respectively. The serum extract was stable when stored for 24h. The developed method was successfully applied to determine PMEA in human serum and urine, and proved suitable to clinical pharmacokinetic study.
PMID: 16765007 [PubMed - in process]
二附院药剂科郭瑞臣
16.Gynecol Oncol. 2006 Oct;103(1):199-206. Epub 2006 Apr 19. Related Articles, Links
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Arsenic trioxide (As(2)O(3)) inhibits peritoneal invasion of ovarian carcinoma cells in vitro and in vivo.
Zhang J, Wang B.
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China.
OBJECTIVES.: To study the role of arsenic trioxide (As(2)O(3)) in regulating peritoneal invasive activity of ovarian carcinoma cells in vitro and in vivo. METHODS.: The effects of As(2)O(3) on human ovarian cancer cell lines (3AO, SW626 and HO-8910PM) migration, invasion and adhesion with tumor cells and human peritoneal mesothelial cells (HPMC) were observed by means of cell migration test, cell invasion test and cell adhesion test. The effects of As(2)O(3) on MMP-2, MMP-9, TIMP-1 and TIMP-2 gene expressions and protein expressions of tumor cells were determined by RT-PCR and ELISA, respectively. In animal experiments, ovarian tumor cells were implanted into abdominal cavity of nude mice and then the nude mice were treated by intraperitoneal injection of different doses As(2)O(3). The foci on the surface of peritoneum were counted. RESULTS.: As(2)O(3) inhibited tumor cells migration, invasion and adhesion with HPMC in a dose-dependent manner, while the same treatment enhanced tumor cell-tumor cell interactions. As(2)O(3) inhibited mRNA and protein expressions of MMP-2, MMP-9 and TIMP-2 of tumor cells. In contrast, As(2)O(3) increased mRNA and protein expressions of TIMP-1. As(2)O(3) could reduce tumor cells peritoneal metastasis in nude mice. CONCLUSION.: As(2)O(3) inhibits in vitro and in vivo peritoneal invasive activity of ovarian carcinoma cells in a dose-dependent manner. Its anti-invasive activity may be the results of reduced cell motility, inhibited attachment of tumor cells to HPMC and enhanced tumor cell-tumor cell interaction, as well as down-regulation of MMP-2 and MMP-9 levels and up-regulation of TIMP-1 level.
PMID: 16624393 [PubMed - in process]
齐鲁妇产科张晶晶，王波
