1: Gynecol Oncol. 2006 Apr 17; [Epub ahead of print]
Arsenic trioxide (As(2)O(3)) inhibits peritoneal invasion of ovarian carcinoma
cells in vitro and in vivo.
Zhang J, Wang B.
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University,
Jinan 250012, Shandong, China.
OBJECTIVES.: To study the role of arsenic trioxide (As(2)O(3)) in regulating
peritoneal invasive activity of ovarian carcinoma cells in vitro and in vivo.
METHODS.: The effects of As(2)O(3) on human ovarian cancer cell lines (3AO,
SW626 and HO-8910PM) migration, invasion and adhesion with tumor cells and human
peritoneal mesothelial cells (HPMC) were observed by means of cell migration
test, cell invasion test and cell adhesion test. The effects of As(2)O(3) on
MMP-2, MMP-9, TIMP-1 and TIMP-2 gene expressions and protein expressions of
tumor cells were determined by RT-PCR and ELISA, respectively. In animal
experiments, ovarian tumor cells were implanted into abdominal cavity of nude
mice and then the nude mice were treated by intraperitoneal injection of
different doses As(2)O(3). The foci on the surface of peritoneum were counted.
RESULTS.: As(2)O(3) inhibited tumor cells migration, invasion and adhesion with
HPMC in a dose-dependent manner, while the same treatment enhanced tumor
cell-tumor cell interactions. As(2)O(3) inhibited mRNA and protein expressions
of MMP-2, MMP-9 and TIMP-2 of tumor cells. In contrast, As(2)O(3) increased mRNA
and protein expressions of TIMP-1. As(2)O(3) could reduce tumor cells peritoneal
metastasis in nude mice. CONCLUSION.: As(2)O(3) inhibits in vitro and in vivo
peritoneal invasive activity of ovarian carcinoma cells in a dose-dependent
manner. Its anti-invasive activity may be the results of reduced cell motility,
inhibited attachment of tumor cells to HPMC and enhanced tumor cell-tumor cell
interaction, as well as down-regulation of MMP-2 and MMP-9 levels and
up-regulation of TIMP-1 level.
PMID: 16624393 [PubMed - as supplied by publisher]
2: Fertil Steril. 2006 Apr;85 Suppl 1:1125-9.
Potential role of toll-like receptor 3 in a murine model of
polyinosinic-polycytidylic acid-induced embryo resorption.
Lin Y, Zeng Y, Zeng S, Wang T.
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou
City, China.
yilinonline@21cn.comOBJECTIVE: To investigate the potential role that toll-like receptor 3 (TLR3)
may play in a murine model of polyinosinic-polycytidylic acid (polyIC)-induced
embryo resorption. DESIGN: An animal model-based study on induced embryo
resorption. SETTING: University animal laboratory. ANIMAL(S): Pathogen-free
animals housed under barrier conditions and monitored for health status.
Pregnant BALB/c mouse mated by C57BL/6 male was used as a model of allogeneic
gestation. INTERVENTION(S): The administration of polyIC was performed to
establish a murine model of induced embryo resorption, with or without TLR3
blocking by multiple injection of mAb against this receptor. MAIN OUTCOME
MEASURE(S): Flow cytometric analysis was performed to detect the percentage of
CD45(+)DX5+ and DX5(+)CD69+ cell subsets, with the DX5 antigen used as a common
natural killer (NK) cell marker, and CD69 as a marker for activated NK cells,
respectively. RESULT(S): In the allogeneic mating model, BALB/cxC57BL/6, both
the CD45(+)DX5+ and DX5(+)CD69+ cell percentages were significantly elevated
upon polyIC stimulation at the absence of anti-TLR3 administration but were kept
unchanged if the female mice were pretreated with anti-TLR3 monoclonal antibody.
Accordingly, the resorption rate of embryos was boosted by polyIC
administration, but this effect could be abrogated by pretreatment of anti-TLR3
mAb. CONCLUSION(S): The engagement of polyIC with TLR3 may be critical to the
activation of NK cells infiltrated at the feto-maternal interface, subsequently
resulting in an increase in embryo resorption.
PMID: 16616084 [PubMed - in process]
3: Gynecol Oncol. 2006 Apr 17; [Epub ahead of print]
Arsenic trioxide (As(2)O(3)) inhibits peritoneal invasion of ovarian carcinoma
cells in vitro and in vivo.
Zhang J, Wang B.
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University,
Jinan 250012, Shandong, China.
OBJECTIVES.: To study the role of arsenic trioxide (As(2)O(3)) in regulating
peritoneal invasive activity of ovarian carcinoma cells in vitro and in vivo.
METHODS.: The effects of As(2)O(3) on human ovarian cancer cell lines (3AO,
SW626 and HO-8910PM) migration, invasion and adhesion with tumor cells and human
peritoneal mesothelial cells (HPMC) were observed by means of cell migration
test, cell invasion test and cell adhesion test. The effects of As(2)O(3) on
MMP-2, MMP-9, TIMP-1 and TIMP-2 gene expressions and protein expressions of
tumor cells were determined by RT-PCR and ELISA, respectively. In animal
experiments, ovarian tumor cells were implanted into abdominal cavity of nude
mice and then the nude mice were treated by intraperitoneal injection of
different doses As(2)O(3). The foci on the surface of peritoneum were counted.
RESULTS.: As(2)O(3) inhibited tumor cells migration, invasion and adhesion with
HPMC in a dose-dependent manner, while the same treatment enhanced tumor
cell-tumor cell interactions. As(2)O(3) inhibited mRNA and protein expressions
of MMP-2, MMP-9 and TIMP-2 of tumor cells. In contrast, As(2)O(3) increased mRNA
and protein expressions of TIMP-1. As(2)O(3) could reduce tumor cells peritoneal
metastasis in nude mice. CONCLUSION.: As(2)O(3) inhibits in vitro and in vivo
peritoneal invasive activity of ovarian carcinoma cells in a dose-dependent
manner. Its anti-invasive activity may be the results of reduced cell motility,
inhibited attachment of tumor cells to HPMC and enhanced tumor cell-tumor cell
interaction, as well as down-regulation of MMP-2 and MMP-9 levels and
up-regulation of TIMP-1 level.
PMID: 16624393 [PubMed - as supplied by publisher]
4: Fertil Steril. 2006 Apr;85 Suppl 1:1125-9.
Potential role of toll-like receptor 3 in a murine model of
polyinosinic-polycytidylic acid-induced embryo resorption.
Lin Y, Zeng Y, Zeng S, Wang T.
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou
City, China.
yilinonline@21cn.comOBJECTIVE: To investigate the potential role that toll-like receptor 3 (TLR3)
may play in a murine model of polyinosinic-polycytidylic acid (polyIC)-induced
embryo resorption. DESIGN: An animal model-based study on induced embryo
resorption. SETTING: University animal laboratory. ANIMAL(S): Pathogen-free
animals housed under barrier conditions and monitored for health status.
Pregnant BALB/c mouse mated by C57BL/6 male was used as a model of allogeneic
gestation. INTERVENTION(S): The administration of polyIC was performed to
establish a murine model of induced embryo resorption, with or without TLR3
blocking by multiple injection of mAb against this receptor. MAIN OUTCOME
MEASURE(S): Flow cytometric analysis was performed to detect the percentage of
CD45(+)DX5+ and DX5(+)CD69+ cell subsets, with the DX5 antigen used as a common
natural killer (NK) cell marker, and CD69 as a marker for activated NK cells,
respectively. RESULT(S): In the allogeneic mating model, BALB/cxC57BL/6, both
the CD45(+)DX5+ and DX5(+)CD69+ cell percentages were significantly elevated
upon polyIC stimulation at the absence of anti-TLR3 administration but were kept
unchanged if the female mice were pretreated with anti-TLR3 monoclonal antibody.
Accordingly, the resorption rate of embryos was boosted by polyIC
administration, but this effect could be abrogated by pretreatment of anti-TLR3
mAb. CONCLUSION(S): The engagement of polyIC with TLR3 may be critical to the
activation of NK cells infiltrated at the feto-maternal interface, subsequently
resulting in an increase in embryo resorption.
PMID: 16616084 [PubMed - in process]
5: Fertil Steril. 2006 Apr;85(4):841.
In vitro oocyte maturation: an important and challenging area for investigation.
Li Y, Mullen SF, Critser JK, Chen ZJ.
Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong
University, Jinan, China.
In vitro maturation of human oocytes has many potential benefits. The
development of this technology relies on results from clinical research, which
has many associated challenges.
PMID: 16580361 [PubMed - in process]
6: Fertil Steril. 2006 Apr;85(4):827-32.
Confocal microscopic analysis of the spindle and chromosome configurations of
human oocytes matured in vitro.
Li Y, Feng HL, Cao YJ, Zheng GJ, Yang Y, Mullen S, Critser JK, Chen ZJ.
Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong
University, Jinan, China.
OBJECTIVE: To assess the potential effects of in vitro maturation (IVM) of human
oocytes on the meiotic spindle and associated chromosome configuration. DESIGN:
Prospective study. SETTING: Hospital-based IVF center. PATIENT(S): Patients with
polycystic ovary syndrome (PCOS) undergoing unstimulated and stimulated cycles
of oocyte retrieval. INTERVENTION(S): Immature (germinal vesicle and metaphase
I) and mature (metaphase II) oocytes were collected from PCOS patients. The
meiotic spindle and chromosome configurations in oocytes matured in vitro and in
vivo were studied by confocal microscopy, with fluorescent labeling techniques
for visualization of both microtubules and chromatin. MAIN OUTCOME MEASURE(S):
Meiotic spindle and associated chromosome configurations. RESULT(S): Oocytes can
develop to the metaphase II stage after IVM. Confocal microscopic observations
revealed that the oocytes matured in vitro had a higher frequency of abnormal
meiotic spindle and chromosomal alignment morphology than in vivo-matured
oocytes. These abnormalities included a partial or total disorganization of the
meiotic spindle microtubules. Abnormal chromosome organization included
dispersal of chromosomes or chromosomes with an aberrant, less-condensed
appearance. The proportions of abnormality in spindle and chromosome
configurations in oocytes matured in vitro were 43.7% and 33.3%, respectively,
which was significantly higher than in those oocytes matured in vivo (13.6% and
9.1%). CONCLUSION(S): In vitro maturation can have deleterious effects on the
organization of the meiotic spindle and chromosome alignment of human oocytes.
This result suggests one possible explanation for the reduced developmental
potential of oocytes matured in vitro compared with those matured in vivo. This
is likely a contributing factor to the overall lower clinical outcomes observed
after IVM and ET.
PMID: 16580356 [PubMed - in process]
7: Neurosci Lett. 2006 Apr 24;397(3):259-62. Epub 2006 Feb 14.
Modulation of the Hering-Breuer reflex by raphe pallidus in rabbits.
Li Y, Song G, Cao Y, Wang H, Wang G, Yu S, Zhang H.
Laboratory of Respiratory Neurobiology, Institute of Physiology, School of
Medicine, Shandong University, Jinan 250012, PR China.
Activation of the pulmonary stretch receptors by lung inflation or vagal
stimulation evokes Hering-Breuer (HB) reflex, which is characterized by
inspiratory inhibition and expiratory prolongation. In this work, whether the HB
reflex could be modulated by the serotonergic raphe pallidus (RP) was studied by
comparing the strength of this reflex before and after electrical or chemical
stimulation of the RP. Experiments were performed on urethane anesthetized adult
rabbits. The HB reflex was simulated with electrical stimulation of the central
end of cervical vagus nerve. The RP was stimulated electrically or chemically by
microinjection of glutamate. We found that after either electrical stimulation
or chemical stimulation of the RP, the inspiratory inhibition and expiratory
prolongation of the HB reflex were significantly attenuated. This attenuation
showed post-stimulation time dependency or short-term memory, as well as RP
stimulation intensity dependency. Results of the present study suggested that
the serotonergic RP could exert its respiratory effects by modulating the
strength of HB reflex.
PMID: 16481107 [PubMed - in process]
8：: J Hum Hypertens. 2006 Apr 20; [Epub ahead of print]
Effect of cilnidipine on left ventricular function in hypertensive patients as
assessed by tissue Doppler Tei index.
Tan HW, Li L, Zhang W, Ma ZY, Zhong XZ, Zhang Y.
[1] 1Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese
Ministry of Education and Chinese Ministry of Public Health, Jinan, China [2]
2Department of Cardiology, QiLu Hospital, Shandong University, Jinan, China.
Tissue Doppler Tei index is pointed to be more effective in the evaluation of
global cardiac function than systolic and diastolic measurements alone in
various heart diseases. This study was designed to assess the effect of
cilnidipine on left ventricular function in hypertensive patients by using this
index. A group of 40 hypertensives (mean age 55+/-8 years, range: 35-65) and 16
controls (mean age 52+/-9 years, range: 36-65) were included. Hypertensives were
classified into non-left ventricular hypertrophy (NLVH) group (25 patients) and
left ventricular hypertrophy (LVH) group (15 patients), and treated with
cilnidipine for 2 months. Before and after treatment, the participants were
examined by echocardiography. Tissue Doppler Tei index was calculated as
diastolic time interval measured from end of late diastole to origin of early
diastole (a') minus systolic S(m) duration (b') divided by b', that is Tei
index=(a'-b')/b'. Thirty-seven hypertensive patients finished the treatment. Tei
index was significantly higher in NLVH and LVH groups than in control group, and
in LVH group than in NLVH group (0.44+/-0.07 vs 0.28+/-0.06, P<0.001;
0.51+/-0.13 vs 0.28+/-0.06, P<0.001; 0.51+/-0.13 vs 0.44+/-0.07, P<0.05). After
treatment, Tei index was significantly decreased (0.40+/-0.11 vs 0.46+/-0.10,
P<0.0001); systolic blood pressure and diastolic blood pressure were also
decreased significantly. In conclusion, Tei index is impaired in hypertensives
before development of ventricular hypertrophy and impairment is more prominent
in hypertrophy. Cilnidipine can improve left ventricular function. Tissue
Doppler Tei index is gaining importance in evaluating LV function after drug
intervention in hypertensive patients.Journal of Human Hypertension advance
online publication, 20 April 2006; doi:10.1038/sj.jhh.1002030.
