检索了一下2005年以后齐鲁发的文章，比原来大发CMJ强了不少了。
1:
Br J Cancer. 2005 Jan 17;92(1):113-9. Enhanced expression of peroxisome proliferator-activated receptor gamma in
epithelial ovarian carcinoma.
Zhang GY, Ahmed N, Riley C, Oliva K, Barker G, Quinn MA, Rice GE.
Department of Obstetrics and Gynaecology, Qilu Hospital of Shandong University,
107 Wenhuaxi Road, Jinan 250012, PR China.
The peroxisome proliferator-activated receptors (PPARs) belong to a subclass of
nuclear hormone receptor that executes important cellular transcriptional
functions. Previous studies have demonstrated the expression of PPARgamma in
several tumours including colon, breast, bladder, prostate, lung and stomach.
This study demonstrates the relative expression of PPARgamma in normal ovaries
and different pathological grades of ovarian tumours of serous, mucinous,
endometrioid, clear cell and mixed subtypes. A total of 56 ovarian specimens
including 10 normal, eight benign, 10 borderline, seven grade 1, nine grade 2
and 12 grade 3 were analysed using immunohistochemistry. Immunoreactive
PPARgamma was not expressed in normal ovaries. Out of eight benign and 10
borderline tumours, only one tumour in each group showed weak cytoplasmic
PPARgamma expression. In contrast, 26 out of 28 carcinomas studied were positive
for PPARgamma expression with staining confined to cytoplasmic and nuclear
regions. An altered staining pattern of PPARgamma was observed in high-grade
ovarian tumours with PPARgamma being mostly localized in the nuclei with little
cytoplasmic immunoreactivity. On the other hand, predominant cytoplasmic
staining was observed in lower-grade tumours. Significantly increased PPARgamma
immunoreactivity was observed in malignant ovarian tumours (grade 1, 2 and 3)
compared to benign and borderline tumours (chi2 = 48.80, P < 0.001). Western
blot analyses showed significant elevation in the expression of immunoreactive
PPARgamma in grade 3 ovarian tumours compared with that of normal ovaries and
benign ovarian tumours (P < 0.01). These findings suggest an involvement of
PPARgamma in the onset and development of ovarian carcinoma and provide an
insight into the regulation of this molecule in the progression of the disease.
PMID: 15583697 [PubMed - indexed for MEDLINE]
2:
Cancer Gene Ther. 2005 Oct 7; [Epub ahead of print] Overexpression of von Hippel-Lindau tumor suppressor protein and antisense
HIF-1alpha eradicates gliomas.
Sun X, Liu M, Wei Y, Liu F, Zhi X, Xu R, Krissansen GW.
[1] 1Department of Molecular Medicine and Pathology, Faculty of Medicine and
Health Science, University of Auckland, Auckland, New Zealand [2] 2Department of
Surgery, Qilu Hospital, Shandong University, Jinan, China.
The von Hippel-Lindau tumor suppressor protein (pVHL) suppresses tumor formation
by binding the alpha subunits of hypoxia-inducible-factors responsible for
stimulating tumor angiogenesis and glycolysis, and targeting them for
ubiquitination and proteasomal destruction. Loss of pVHL leads to tumorigenesis
and development of sporadic renal cell carcinomas and central nervous system
hemangioblastomas. In the present study, we investigated whether engineered
overexpression of pVHL in C6 glioma cells, which already express endogenous
pVHL, would suppress the tumorigenicity of this particular tumor cell type. C6
cells overexpressing VHL displayed a reduced growth rate (70% inhibition)
compared to the parental cell line when subcutaneously implanted in athymic
(nu/nu) mice. Growth inhibition was associated with a 50% reduction in the
number of tumor vessels and a 60% increase in tumor cell apoptosis, due in part
to downregulation of HIF-1, VEGF, and the antiapoptotic factor Bcl-2,
respectively. Gene transfer of VHL suppressed the growth of established C6
gliomas, and synergized with antisense HIF-1 to completely eradicate tumors. The
data suggest that VHL gene therapy and/or agents that increase VHL expression
could have utility in the treatment of gliomas, particularly when combined with
agents that inhibit the expression or function of HIF-1.Cancer Gene Therapy
advance online publication, 7 October 2005; doi:10.1038/sj.cgt.7700907.
PMID: 16211089 [PubMed - as supplied by publisher]
3:
Cancer Gene Ther. 2005 Jan;12(1):35-45. Intramuscular delivery of antiangiogenic genes suppresses secondary metastases
after removal of primary tumors.
Sun X, Qiao H, Jiang H, Zhi X, Liu F, Wang J, Liu M, Dong D, Kanwar JR, Xu R,
Krissansen GW.
Department of Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
k.sun@auckland.ac.nzThe success of surgery to remove primary tumors can be compromised by the
subsequent outgrowth of metastases. It is recognized that primary tumors secrete
antiangiogenic factors that suppress the outgrowth of their daughter metastases.
In accord we show here that surgical removal of primary EL-4 lymphomas led to a
marked decrease in the levels of circulating angiostatin and endostatin, and
promoted the growth of distant nodular tumors. Expression vectors encoding
angiostatin and endostatin, formulated with poly-N-vinyl pyrrolidone (PVP), were
injected into the tibialis and gastrocnemia muscles, leading to expression of
angiostatin and endostatin in muscle fibers. High levels of biologically active
exogenous proteins were secreted into the circulation. Intramuscular gene
therapy with angiostatin and endostatin plasmids significantly inhibited tumor
vascularity and induced tumor cell apoptosis, and thereby suppressed the growth
of secondary subcutaneous and disseminated metastatic tumors in the lung and
liver. Simultaneous intramuscular delivery of both angiostatin and endostatin
plasmids significantly prolonged the survival of mice after removal of primary
tumors. These results suggest that intramuscular gene transfer of angiostatin
and endostatin might serve as a prophylactic cancer-prevention strategy to
combat the recurrence of cancer after surgical resection of primary tumors.
PMID: 15486558 [PubMed - indexed for MEDLINE]
4:
Chin Med J (Engl). 2005 May 20;118(10):854-6. Relations of nuclear factor-kappa B activity in the kidney of children with
primary nephrotic syndrome to clinical manifestations, pathological types, and
urinary protein excretion.
Zhao HY, Sun RP, Dong JH, Zhen JH.
Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012,
China.
liyumei781210@yahoo.com.cnPMID: 15989768 [PubMed - indexed for MEDLINE]
5:
Chin Med Sci J. 2005 Sep;20(3):206-9. Three-dimensional computed tomography-guided radiofrequency trigeminal rhizotomy
for treatment of idiopathic trigeminal neuralgia.
Liu M, Wu CY, Liu YG, Wang HW, Meng FG.
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012.
liumengqilu@vip.sina.comOBJECTIVE: To evaluate the effectiveness of three-dimensional computed
tomography (3D-CT) guided radiofrequency trigeminal rhizotomy (RF-TR) in
treatment of idiopathic trigeminal neuralgia (ITN). METHODS: From 1999 to 2001,
18 patients with ITN were treated with percutaneous controlled RF-TR.
Intraoperative 3D-CT scanning was performed to guide the trajectory of the
puncture. After correction of the needle tip according to the CT scans and
stimulation effects, 2 to 5 lesions were made for a duration of 60-90 seconds at
a temperature of 60 degrees C to 75 degrees C depending on the pain distribution
and the age of patient. RESULTS: The needles located in foramen ovale. Pain
alleviated immediately with no serious complication in all patients. The
patients were followed up for an average of 31.5 months (range 24-41 months).
Acute pain relief was experienced by 17 patients after the procedure, reaching
an initial success rate of 94.4%. Early (< 6 months) pain recurrence was
observed in 2 patients (11.1%), whereas late (> 6 months) recurrence was
reported in 3 patients (16.7%). Thirteen patients had complete pain control,
with no need for medication thereafter. Five cases experienced partial pain
relief, but required medication at a lower dose than in the preoperative period.
CONCLUSION: 3D-CT foramen ovale locations can raise the successful rate of
puncture, enhance the safety, and reduce the incidence rate of complication.
PMID: 16261895 [PubMed - in process]
6:
East Afr Med J. 2005 Jan;82(1):28-33. Complex therapy for hepatic trauma.
Fengjun L, Mteta KA, Xuting Z, Nanhai S.
General Surgery Department, Qilu Hospital of Shandong University, Jinan, China
250012.
OBJECTIVE: To investigate the optimal surgical management of patients with
hepatic trauma. DESIGN: A retrospective analysis of 197 patients treated for
hepatic trauma in the two hospitals from January 1980 to January 2001. SETTING:
Qilu and Dodoma Hospital in China and Tanzania respectively. PATIENT
INTERVENTIONS: Two patients died before surgery, seven patients were treated
conservatively, while 188 patients underwent various surgical interventions
under the principles of damage control surgery including initial laparotomy,
resuscitation phase and definitive surgery. RESULTS: The overall mortality was
15.3% (30/197). The leading cause of death was the triad of coagulopathy,
hypothermia and metabolic acidosis. CONCLUSION: Patients with major
exanguinating injuries will not survive complex procedures such as formal
hepatic resection or complex procedures such as formal hepatic resection or
pancreaticoduodenectomy. The operating team must undergo a radical shift in
their "surgical ideology" if the patient is to survive such devastating
injuries. The central principle of damage control surgery is that patients died
of the triad of coagulopathy, hypothermia and metabolic acidosis. Damage control
surgery can be thought of in three distinct phases: initial truncated
laparotomy, resuscitation phase and definitive operation.
PMID: 16122109 [PubMed - indexed for MEDLINE]
7:
Eur J Haematol. 2005 Nov 18; [Epub ahead of print] Effects of IgG and its F(ab') fragments of some patients with idiopathic
thrombocytopenic purpura on platelet aggregation.
Chu XX, Hou M, Peng J, Zhu YY, Ji XH, Wang L, Zhang F, Ma DX.
Hematology Oncology Center, Qilu Hospital of Shandong University, Jinan,
Shandong, China.
Objectives: To make humanized monoclonal antibodies by phage surface display
technology, we screened out the specific anti-platelet glycoproteins (GPs) IgG
antibody from patients with chronic idiopathic thrombocytopenic purpura (ITP),
which can inhibit platelet aggregation. Methods: We studied plasmas from 68
patients with ITP for the presence of IgG antibodies specific for GPIIb/IIIa
and/or GPIb/IX using modified monoclonal antibody immobilization of platelet
antigen assays. The IgG antibody and its F(ab')(2) fragments of the positive
plasmas which could inhibit platelet aggregation function were prepared and
purified. Their immunoreactivity to platelet GPs and effects on platelet
function were further analyzed.Results: GPIIb/IIIa- and GPIb/IX-specific
antibodies were found in 21 and 19 patients, respectively. Six of them had
antibodies against both GP complexes. Among the 34 positive plasmas, four with
positive anti-GPIIb/IIIa autoantibody showed significant inhibition of platelet
aggregation induced by adenosine diphosphate (ADP), whereas one with
GPIb/IX-specific antibody inhibited ristocetin-induced platelet aggregation. The
purified IgG and its F(ab')(2) fragments from two patients not only retained the
ability to bind to platelet GPs but also impaired the in vitro ADP-induced
platelet aggregation.Conclusions: F(ab')(2) portion of the IgG is a functional
fragment, which is responsible for the autoantibody interaction with platelet
GPs in ITP, and some of them also affect platelet function, which can be used to
develop completely humanized anti-GPIIb/IIIa small molecular phage antibody.
PMID: 16293117 [PubMed - as supplied by publisher]
8:
Immunogenetics. 2005 Feb;56(11):781-7. Epub 2005 Jan 14. HLA polymorphisms are associated with Helicobacter pylori infected gastric
cancer in a high risk population, China.
Li Z, Chen D, Zhang C, Li Y, Cao B, Ning T, Zhao Y, You W, Ke Y.
Department of General Surgery, Qilu Hospital of Shandong University, Jinan,
People's Republic of China.
Helicobacter pylori is one of the most common bacterial infections associated
with an increased risk of gastric cancer, but its association with host factors,
particularly polymorphisms of the immune response genes, such as human leukocyte
antigen (HLA) genes, is still unclear. To investigate the role of HLA
polymorphisms in the risk of gastric cancer among subjects with H. pylori
infection, a case-control study involving 52 gastric cancer patients and 139
non-cancer controls was conducted in Linqu County, China, an area with a high
incidence of gastric cancer. Polymorphisms of HLA class I and class II alleles
were determined by PCR with sequence-specific primers (PCR-SSP). The information
about H. pylori infection was obtained from previous records. Among 48 class I
and 19 class II HLA alleles detected in this study, two alleles, CW*03 and
DRB1*01, were found to be distributed significantly differently between patients
and controls [odds ratio(OR)=1.95, 95% confidence interval (CI)=1.13-3.35,
P=0.017 and OR=4.39, 95% CI=1.39-13.84, P=0.012, respectively). The OR of
gastric cancer risk in individuals carrying CW*03/CW*03 or CW*03/CW*N was 2.06,
95% CI=1.05-4.02, P=0.035, while the OR was 3.49, 95% CI=1.0-12.4, P=0.04 for
DRB1*01/DRB1*01 or DRB1*01/DRB1*N carriers. The analysis of the interaction
between H. pylori infection and HLA risk genotypes of CW*03 or DRB1*01 revealed
that the effect of CW*03 and DRB1*01 genotypes on gastric cancer risk was
manifested stronger in H. pylori-positive individuals (OR=5.30, 95%
CI=1.73-16.29, P=0.004 and OR=13.38, 95% CI=2.52-70.98, P=0.002, respectively)
than in H. pylori-negative ones (OR=1.25, 95% CI=0.25-6.12, P=0.785 and OR=2.26,
95% CI=0.18-28.88, P=0.531, respectively). The combined effect of the two risk
HLA genotypes on gastric cancer risk was also analysed. The result showed that
the individuals carrying both the CW*03 and DRB1*01 alleles could only be found
in cancer patients (5/52), and not in controls (0/139), further suggesting that
CW*03 and DRB1*01 are risk alleles advancing the progression of tumorigenesis.
These observations demonstrate that host HLA genotypes may play an important
role in the risk of gastric cancer, especially among persons with H. pylori
infection.
PMID: 15650879 [PubMed - indexed for MEDLINE]
9:
Immunol Cell Biol. 2005 Dec;83(6):668-73. Hypoxia inhibits the migratory capacity of human monocyte-derived dendritic
cells.
Qu X, Yang MX, Kong BH, Qi L, Lam QL, Yan S, Li P, Zhang M, Lu L.
Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan,
China.
Hypoxia, a prominent characteristic of inflammatory tissue lesions and solid
tumour microenvironments, is a crucial stimulus capable of modulating the
expression of specific genes involved in leucocyte recruitment. Although studies
have shown that hypoxia can affect leucocyte migration by influencing the
expression of migration-related genes, such as matrix metalloproteinases (MMP)
and their endogenous tissue inhibitors of matrix metalloproteinases (TIMP), it
remains unclear whether hypoxia can affect the migration of dendritic cells
(DC). In this study, we showed that human monocyte-derived DC under hypoxic
conditions in a transwell system have significantly reduced migratory capacity
compared to normoxic controls. A moderate phenotypic change of hypoxic DC was
observed. In hypoxic DC, we detected a twofold increase in TIMP-1 transcript
levels, and downregulated expression of MMP-9 and membrane type 1-MMP genes by
threefold and 17-fold, respectively. Our results suggest that hypoxia may
inhibit DC migratory activity by regulating the balance between MMP and TIMP
gene expression.
PMID: 16266319 [PubMed - indexed for MEDLINE]
10:
J Chemother. 2005 Jun;17(3):302-8. High dose chemotherapy and transplantation of hematopoietic progenitors from
murine D3 embryonic stem cells.
Jiang J, Kong B, Shen B, Li L, Yang X, Hou H, Shi Q, Ma D, Ma X.
Qilu Hospital, Shandong University, Jinan, China.
Differentiating embryonic stem (ES) cells are an increasingly important source
of hematopoietic progenitors, useful for both basic research and clinical
applications. To date, characteristics of specific factors capable of
influencing hematopoietic cell fate from ES cells remains elusive. We report
that mMSC Feeder Layer and the combination of VEGF, SCF and TPO strongly promote
hematopoietic differentiation. The results showed that the cells induced from
ES-D3 expressed hematopoietic progenitor antigens (CD34 and CD117), myelocyte
cell antigen (CD11b), erythrocyte cell antigen (Ter119), and transcription
factors (Flk-1, GATA-2, SCL, beta-H1 and beta-major). Furthermore, those induced
differentiated cells were injected into female C57BL/6 mice which were treated
with high dose topotecan chemotherapy to restore part of their blood system
function. We observed rapid white blood cell recovery, which suggested that the
infusion of differentiated cells has a positive impact on hematopoiesis. The Sry
gene in peripheral blood, bone marrow and spleen of transplanted female mice was
confirmed by PCR analysis, which affirmed the existence of the chimera.
PMID: 16038524 [PubMed - indexed for MEDLINE]
11:
J Clin Neurosci. 2005 Sep;12(7):784-6. Cerebellopontine angle epidermoids presenting with trigeminal neuralgia.
Meng L, Yuguang L, Feng L, Wandong S, Shugan Z, Chengyuan W.
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, PR
China.
We studied the clinical characteristics of cerebellopontine angle (CPA)
epidermoids presenting with trigeminal neuralgia (TN). Twenty-four patients were
analyzed retrospectively and the literature reviewed with regard to clinical
manifestation, imaging features, surgical procedures and prognosis. TN may be
the initial symptom of CPA epidermoid, particularly in young patients.
Epidermoids are characteristically hypodense nonenhancing lesions on CT scans,
while on MRI they exhibit long T1 and T2 relaxation times. Although complete
removal is ideal in the surgical management of CPA epidermoid, proximity to
cranial nerves and the brain stem may pose technical difficulties in complete
resection. In addition to complete resection of the tumour, arterial compression
at the root entry zone (REZ) of the trigeminal nerve should be sought, and if
found, a microvascular decompression (MVD) should be performed. Cranial nerve
dysfunction and aseptic meningitis are the most common operative complications.
PMID: 16150598 [PubMed - in process]
12:
J Clin Neurosci. 2005 Apr;12(3):256-60. Neuroendoscopic anatomy and surgery of the cerebellopontine angle.
Yuguang L, Chengyuan W, Meng L, Shugan Z, Wandong S, Gang L, Xingang L.
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, PR
China.
To probe the feasibility and utility of neuroendoscopic inspection of the
anatomy of the cerebellopontine angle (CPA) and of neuroendoscopic assisted
microneurosurgery (NEAMN) for CPA lesions via a retrosigmoid approach, we used
retrosigmoid NEAMN in 28 patients with CPA lesions. Prior to this, we undertook
anatomical observation of bilateral CPA in two adult cadaver heads using the
neuroendoscope. NEAMN tumour resection was performed in eight acoustic neuromas,
one meningioma and 14 cholesteatomas and NEAMN vascular decompression was
performed in five patients with trigeminal neuralgia. Both the neurovascular
structures of the CPA and the ventral surface of the pons, as well as the
clivus, can be inspected using the neuroendoscope through a retrosigmoid
approach with a 2-3 cm diameter bony opening. Complete excision of the tumour
with preservation of the facial nerve was achieved in all eight acoustic
neuromas. Likewise, total resection of the tumour was possible in the 14
cholesteatomas and one meningioma. Paroxysmal facial pain resolved after NEAMN
vascular decompression in the five patients with trigeminal neuralgia. There
were no postoperative complications or deaths in this series. The CPA can be
divided into three levels - the cranial, medial, and caudal, and each level
contains specific neurovascular structures as seen through the neuroendoscope.
Knowledge of these divisions is useful to master the common NEAMN procedures of
the CPA. NEAMN for CPA lesions via a retrosigmoid approach is a useful adjunct
to standard microneurosurgical techniques effect and may decrease the operative
risk.
PMID: 15851077 [PubMed - in process]
13:
J Clin Neurosci. 2005 Apr;12(3):253-5. Cystic acoustic neuroma.
Wandong S, Meng L, Xingang L, Yuguang L, Shugan Z, Lei W, Chengyuan W.
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, PR
China.
To define the clinical characteristics of cystic acoustic neuroma, we
retrospectively analyzed 22 patients with cystic acoustic neuroma and reviewed
the literature with regard to clinical manifestation, imaging features,
diagnosis, surgical procedures and prognosis. An acoustic neuroma was defined as
cystic according to the following criteria: the presence of
hypodense/hypointense areas on CT or MRI, the identification of cystic elements
at operation and histological verification. At the end of surgery, the facial
nerve was anatomically intact in 86.4% of cystic acoustic neuromas. Complete
removal of the tumor was achieved in 18 cases (81.8%). We conclude that patients
with cystic acoustic neuroma need prompt surgery with special attention paid to
the preservation of the facial nerve.
PMID: 15851076 [PubMed - in process]
14:
J Long Term Eff Med Implants. 2005;15(4):375-88. Management of gastroesophageal reflux disease: medications, surgery, or
endoscopic therapy? (Current status and trends).
Zhi XT, Kavic SM, Park AE.
Department of General Surgery, Qilu Hospital, Shandong University, Jinan,
Shandong, P. R. China.
Gastroesophageal reflux disease (GERD) is a common chronic disorder in the
Western world. The basic cause of GERD has been well characterized--the
fundamental defect is a loss of integrity of the gastroesophageal barrier. What
is less clear is the most appropriate means of addressing this reflux. GERD has
a variety of symptoms, ranging from typical presentations of heartburn and
regurgitation (without esophagitis) to atypical presentations, such as severe
erosive esophagitis and its associated complications. Because of its symptomatic
diversity, physicians may select from a variety of therapeutic approaches.
Medical therapy aims at decreasing acidity by suppressing proton secretion and
has been well established. Available medications include antacids and alginates,
H2-receptor antagonists, motility agents, and proton pump inhibitors (PPIs).
Antireflux surgery, commonly performed laparoscopically, aims at reinforcing and
repairing the defective barrier through plication of the gastric fundus. The
earliest performed successful procedures were the Nissen and Toupet
fundoplications, to which several modifications have since been made. It has
been demonstrated in preliminary studies and long-term outcomes of such open
surgery and preliminary studies of such laparoscopic surgery that antireflux
surgery is an effective approach, with overall outcomes superior to those
achieved with medications. The precise indications for the surgical treatment of
patients with GERD, however, remain controversial. In recent years, endoscopic
intraluminal antireflux approaches have attracted the attention of physicians,
surgeons, and commercial companies, especially after the approval of two
endoscopic intraluminal methods by the United States FDA in 2000. The common
element is prevention of acid reflux by construction of a functional or
controlled barrier in the lower esophageal sphincter zone. Three main methods
are currently employed: endoscopic intraluminal valvuloplasty, endoscopic
radiofrequency therapy, and endoscopic injection or implantation of foreign
material. The endoluminal suturing method is highly demanding technically, and
its short-term results are encouraging, although largely dependent on the
experience of the endoscopist. Several prospective cohort studies have shown
that the radiofrequency procedure (Stretta) significantly improves GERD symptoms
and quality of life while reducing esophageal acid exposure and eliminating the
need for antisecretory medications in the majority of patients within 6-12
months. Most recently, some researchers have studied the endoluminal
implantation of polymers, such as Plexiglas (polymethyl-methylacrylate),
Gatekeeper hydrogel, and Enteryx (ethylene vinyl alcohol copolymer). The
preliminary results of these studies showed that the implantation method was
feasible and safe; however, the only multicenter trial related to outcome that
has been published has included just 1 year of follow-up. Here, we review the
treatment of GERD: medical, surgical, and endoscopic. In addition, we provide an
algorithm based on symptoms and response to treatment for management of these
patients.
Publication Types:
Review
PMID: 16022648 [PubMed - indexed for MEDLINE]
15:
J Pharm Biomed Anal. 2005 Nov 26; [Epub ahead of print] Validated LC/MS/MS assay for curcumin and tetrahydrocurcumin in rat plasma and
application to pharmacokinetic study of phospholipid complex of curcumin.
Liu A, Lou H, Zhao L, Fan P.
School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012,
PR China; Qilu Hospital of Shandong University, Jinan, Shandong 250012, PR
China.
To study pharmacokinetic properties of curcumin, a fast sensitive assay method
was developed to determine curcumin and its metabolite tetrahydrocurcumin in rat
plasma. The assay was based on tandem mass spectrometry detection (LC/MS/MS).
Salbutamol was used as the internal standard (IS). The method had the lower
limit of quantitation (LLOQ) of 0.5ng/ml in rat plasma, which corresponds to
2.5pg for the 5mul injection volume. Good linearity was got to 500ng/ml. The
precision, accuracy, recovery and applicability were found to be adequate for
pharmacokinetic studies. Phospholipid complex of the natural compound curcumin
was prepared in order to improve its bioavailability. Complex formation resulted
in an obvious increase in bioavailability of curcumin in rat in vivo according
to the assay by above LC/MS/MS method.
PMID: 16316738 [PubMed - as supplied by publisher]
16:
J Ultrasound Med. 2006 Jan;25(1):7-14. Gray scale contrast enhancement and quantification in different positions of
rabbit liver.
Li J, Dong BW, Yu XL, Li CF.
Department of Ultrasound, Qilu Hospital, Shandong University, School of
Medicine, Jinan 250012, China.
jieli301@hotmail.com.
OBJECTIVE: The purpose of this study was to evaluate the difference between
central and ventral peripheral positions and the difference between left and
right lobes in rabbit liver with gray scale contrast enhancement. METHODS: An in
vivo model of perfusion was studied with a sulfur hexafluoride contrast agent
and low-mechanical-index, real-time, gray scale harmonic imaging. The contrast
agent (0.1 mL/kg body weight) was applied respectively in 10 rabbits by
intravenous bolus injection. The time-intensity curve was used to obtain
flow-related parameters such as time to enhancement (ET), time to peak intensity
(PIT), peak signal intensity (PSI), enhancement duration (ED), and area under
the curve (AUC). RESULTS: There was a significant difference in parameters of
the time-intensity curve between central and peripheral ventral positions of
liver parenchyma (P < .05), except for the ED in the left liver. The ET and PIT
were earlier, the PSI higher, the ED longer, and the AUC larger in the central
position of parenchyma than in the peripheral position. In addition, the ET and
PIT were earlier, the PSI higher, the ED longer, and the AUC larger in the right
lobe of liver parenchyma than in the left lobe. There was a significant
difference in parameters of the time-intensity curve between the left and right
lobes of liver parenchyma (P < .05), except for the ET of the peripheral
position. CONCLUSIONS: Flow parameters are different between central and ventral
peripheral positions and between left and right lobes of hepatic parenchyma.
PMID: 16371550 [PubMed - in process]
17:
J Ultrasound Med. 2005 Jul;24(7):975-83. Time-intensity-based quantification of vascularity with single-level dynamic
contrast-enhanced ultrasonography: a pilot animal study.
Li J, Dong BW, Yu XL, Wang XH, Li CF.
Department of Ultrasound, Qilu Hospital, Shandong University, School of
Medicine, Jinan 250012, China.
jieli301@hotmail.comOBJECTIVE: The purpose of this study was to delineate the hemodynamic features
of VX2 tumor and perineoplastic liver parenchyma and to evaluate the potential
usefulness of single-level dynamic ultrasonography in the diagnosis of tumors by
the analysis of time-intensity curves. METHODS: An in vivo animal model was
studied using a low mechanical index in conjunction with single-level dynamic
contrast-enhanced ultrasonography. A sulfur hexafluoride contrast agent
(SonoVue; Bracco SpA, Milan, Italy) was applied in 8 rabbits by intravenous
bolus injection. Data were acquired before and after VX2 tumor induction.
Corresponding parameters of the time-intensity curve were measured using
wash-in/wash-out curve software. RESULTS: No significant difference was found in
the time to enhancement, time to peak intensity, peak signal intensity, and
enhancement duration between liver parenchyma before and after VX2 tumor
induction (P > .05). The typical enhancement pattern of VX2 tumors was
hyperechoic relative to liver parenchyma during the early phase and hypoechoic
during the later phase. The curves obtained in carcinomas revealed an early
arrival time and time to peak intensity with an irregular and sharp decrease of
the intensity signal and a very early return to baseline, presenting a much more
rapid wash-in and wash-out of ultrasonographic contrast agents. There was a
significant difference in the time to enhancement, time to peak intensity, peak
signal intensity, and enhancement duration between the VX2 tumors and
perineoplastic liver parenchyma (P < .001). CONCLUSIONS: Single-level dynamic
contrast-enhanced ultrasonography with a low mechanical index level could
provide real-time and continuous enhanced images and fully delineate the typical
enhancement pattern of liver tumors. The analysis of time-intensity curves may
provide useful, complementary, and quantitative information. This technique may
be useful for the diagnosis of liver tumors, especially those showing an
atypical enhancement pattern on biphasic helical computed tomographic scanning.
PMID: 15972712 [PubMed - in process]
18:
Neurosurg Rev. 2006 Jan;29(1):36-40. Epub 2005 Oct 12. Intraventricular meninigiomas: a report of 25 cases.
Liu M, Wei Y, Liu Y, Zhu S, Li X.
Department of Neurosurgery, Qilu Hospital of Shandong University, Wenhua Xi Road
107#, Jinan 250012, People's Republic of China.
liumengqilu@vip.sina.comIn order to study the clinical characteristics of intraventricular meningiomas,
we analyzed retrospectively 25 patients and reviewed the literature with regard
to incidence, clinical manifestation, imaging features, preoperative diagnosis,
surgical findings, and histopathological results. Intraventricular meningiomas
are quite rare, but they represent an important differential diagnosis of
intraventricular neoplasms. Computed tomography or magnetic resonance imaging
enable a correct diagnosis of intraventricular meningiomas in most of the cases.
The tumors often grow slowly to a substantial size before they become
symptomatic. The operative route should be selected according to the tumor's
location. Out of the 24 lateral ventricular meningiomas in our series, 20 were
resected via a posterior parieto-occipital transcortical approach, two were
resected via a transcallosal approach, and another two tumors, located in the
frontal horn and body of the lateral ventricle, were resected via a frontal
approach. A median suboccipital craniotomy was performed for the fourth
ventricular meningioma. The parieto-occipital route for lateral ventricular
meningiomas is a safe surgical approach, which is not necessarily associated
with postoperative visual deficits. Piecemeal removal of the tumor can be safely
and easily performed and special attention should be paid to the choroidal
vessels intraoperatively.
PMID: 16220350 [PubMed - in process]
19:
Ultrasound Med Biol. 2005 Feb;31(2):185-90. Grey-scale contrast enhancement in rabbit liver with SonoVue at different doses.
Li J, Dong B, Yu X, Wang X, Li C.
Department of Ultrasound, Qilu Hospital, Shandong University, Jinan, China.
To evaluate the dose of ultrasound (US) contrast agent (UCA) in relation to the
contrast-enhancement effect, an in vivo model of perfusion was studied using
SonoVue, a second-generation UCA, and low mechanical index (MI) grey-scale
harmonic imaging. SonoVue, at eight different doses (0.02, 0.04, 0.06, 0.08,
0.10, 0.12, 0.14 and 0.16 mL/kg BW), was applied in five normal rabbits.
Flow-related parameters obtained from time-intensity curves were calculated and
plotted over the contrast agent doses, and nonlinear curve fitting was
performed. Results showed that, along with an increase of the administrated
contrast agent dose, the enhancement duration (ED) and the area under the curve
(AUC) increased logarithmically, and the time to enhancement (ET) decreased
logarithmically. There was a progressive increase of the peak signal intensity
(PSI) following an increase of SonoVue dose only in the dose range of 0.02 up to
0.10 mL/kg body weight (BW) in the portal vein and in the dose range of 0.02 up
to 0.12 mL/kg BW in the liver parenchyma. Moreover, a good correlation was
observed between the parameters obtained from liver parenchyma and those
obtained from the portal vein. The results indicated that SonoVue in conjunction
with continuous harmonic low-MI grey-scale imaging has the capability of flow
quantification on both vessels and parenchyma. The parameters of time-intensity
curve were influenced intensely by different contrast agent doses.
PMID: 15708457 [PubMed - indexed for MEDLINE]
20:
World J Gastroenterol. 2005 Oct 7;11(37):5757-62. Polymorphism of CYPIA1 and GSTM1 genes associated with susceptibility of gastric
cancer in Shandong Province of China.
Li H, Chen XL, Li HQ.
Center of Tumour Treatment, Qilu Hospital of Shandong University, Jinan 250012,
Shandong Province, China.
haoli611@yahoo.comAIM: To explore whether polymorphisms of the CYPIA1 and GSTM1 genes are
associated with susceptibility of stomach cancer. METHODS: A total of 102
stomach cancer cases and 62 healthy persons were diagnosed by pathology in
1998-2000 in the Qilu Hospital of Shandong University. Gene polymorphisms were
detected by the PCR using sequence-specific primers. Data analysis of the
case-control study was carried out using the unconditional logistic method.
RESULTS: After adjustment for age, sex, educational levels, and occupation, the
risk factors for stomach cancer were shown to be smoking, Helicobacter pylori (H
pylori), and presence of the CYPIM G/G and GSTM1 O/O genotypes. Interaction was
observed between the combined genotypes of either CYPIA1 G/G and GSTM1 O/O or H
pylori infection, or GSTM1 O/O and H pylori infection or smoking. CONCLUSION:
Polymorphisms of the CYPIA1 and GSTM1 genes, H pylori infection and smoking are
related to susceptibility to stomach cancer.
PMID: 16270381 [PubMed - indexed for MEDLINE]
21:
World J Gastroenterol. 2005 Aug 14;11(30):4753-7. Influence of HLA-DRB1 alleles and HBV genotypes on interferon-alpha therapy for
chronic hepatitis B.
Chu RH, Ma LX, Wang G, Shao LH.
Department of Infectious Diseases, Qilu Hospital of Shandong University, Jinan,
Shandong Province, China.
rhchu@163.comAIM: To investigate the influence of HLA-DRB(1) alleles and HBV genotypes on
interferon-alpha therapy for chronic hepatitis B. METHODS: HLA-DRB1*03, *07,
*09, *12, *15 alleles were determined using polymerase chain reaction/sequence
specific primer (PCR/SSP) technique in 126 patients with chronic hepatitis B and
76 normal control subjects in Shandong Province, and HBV genotypes were
determined by nested-PCR analysis using type-specific primers in 126 patients.
RESULTS: The positivity of HLA-DRB1*07 allele in chronic hepatitis B group was
significantly higher than that in normal control group (chi(2) = 6.33, P<0.025,
RR = 2.37). Among the 126 patients, genotype B was found in 38 (30.2%), genotype
C in 69 (54.8%), and mixed genotype (B+C) in 19 (15.0%), genotypes D-F were not
found. Among the 46 DRB1*07(+) patients, 7 were responders and 39 were
non-responders among them (chi(2) = 6.71, P<0.05). The positivity of HLA-DRB1*07
and prevalence of HBV genotype C were significantly higher in non-responders
than in responders. CONCLUSION: High positivities of HLA-DRB1 *07 allele and HBV
genotype C are closely associated with the lower response to interferon-alpha
therapy for chronic hepatitis B.
PMID: 16094724 [PubMed - indexed for MEDLINE]
22:
World J Gastroenterol. 2005 Jul 14;11(26):4003-7. Radiosensitivity of human colon cancer cell enhanced by immunoliposomal
docetaxel.
Wang QW, Lu HL, Song CC, Liu H, Xu CG.
Cancer Research Center, Qilu Hospital of Shandong University, Jinan 250012,
Shandong Province, China.
wangqingwei@csco.org.cnAIM: To enhance the radiosensitivity of human colon cancer cells by docetaxel.
METHODS: Immunoliposomal docetaxel was prepared by coupling monoclonal antibody
against carcinoembryonic antigen to cyanuric chloride at the PEG terminus of
liposome. LoVo adenocarcinoma cell line was treated with immunoliposomal
docetaxel or/and irradiation. MTT colorimetric assay was used to estimate
cytotoxicity of immunoliposomal docetaxel and radiotoxicity. Cell cycle
redistribution and apoptosis were determined with flow cytometry. Survivin
expression in LoVo cells was verified by immunohistochemistry. D801 morphologic
analysis system was used to semi-quantify immunohistochemical staining of
survivin. RESULTS: Cytotoxicity was induced by immunoliposomal docetaxel alone
in a dose-dependent manner. Immunoli-posomal docetaxel yielded a cytotoxicity
effect at a low dose of 2 nmol/L. With a single dose irradiation, the relative
surviving fraction of LoVo cells showed a dose-dependent response, but there
were no significant changes as radiation delivered from 4 to 8 Gy. Compared with
liposomal docetaxel or single dose irradiation, strongly radiopotentiating
effects of immunoliposomal docetaxel on LoVo cells were observed. A low dose of
immunoliposomal docetaxel could yield sufficient radiosensitivity.
Immunoliposomal docetaxel were achieved both specificity of the conjugated
antibody and drug radiosensitization. Combined with radiation, immunoliposomal
docetaxel significantly increased the percentage of G(2)/M cells and induced
apoptosis, but significantly decreased the percentage of cells in G(2)/G(1) and
S phase by comparison with liposomal docetaxel. Immunohistochemical analysis
showed that the brown stained survivin was mainly in cytoplasm of LoVo cells.
Semi-quantitative analysis of the survivin immunostaining showed that the
expression of survivin in LoVo cells under irradiation with immunoliposomal
docetaxel was significantly decreased. CONCLUSION: Immunoliposomal docetaxel is
strongly effective for target radiosensitation in LoVo colon carcinoma cells,
and may offer the potential to improve local radiotherapy.
PMID: 15996023 [PubMed - indexed for MEDLINE]
23:
World J Gastroenterol. 2005 Jun 21;11(23):3605-9. Effects of L-arginine on serum nitric oxide, nitric oxide synthase and mucosal
Na+-K+-ATPase and nitric oxide synthase activity in segmental small-bowel
autotransplantation model.
Fu TL, Zhang WT, Chen QP, Gao Y, Hu YH, Zhang DL.
Department of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan,
Shandong Province, China.
AIM: To explore a simple method to create intestinal autotransplantation in rats
and growing pigs and to investigate the effect of L-arginine supplementation on
serum nitric oxide (NO), nitric oxide synthase (NOS) and intestinal mucosal NOS
and Na+-K+-ATPase activity during cold ischemia-reperfusion (IR) in growing
pigs. METHODS: In adult Wistar rat models of small bowel autotransplantation, a
fine tube was inserted into mesenteric artery via the abdominal aorta. The
superior mesenteric artery and vein were occluded. Isolated terminal ileum
segment was irrigated with Ringer's solution at 4 degrees and preserved in the
same solution at 0-4 degrees for 60 min. Then, the tube was removed and
reperfusion was established. In growing pig models, a terminal ileum segment, 50
cm in length, was isolated and its mesenteric artery was irrigated via a needle
with lactated Ringer's solution at 4 degrees. The method and period of cold
preservation and reperfusion were described above. Ten white outbred pigs were
randomly divided into control group and experimental group. L-arginine (150
mg/kg) was continuously infused for 15 min before reperfusion and for 30 min
after reperfusion in the experimental group. One, 24, 48, and 72 h after
reperfusion, peripheral vein blood was respectively collected for NO and NOS
determination. At the same time point, intestinal mucosae were also obtained for
NOS and Na+-K+-ATPase activity measurement. RESULTS: In adult rat models, 16 of
20 rats sustained the procedure, three died of hemorrhage shock and one of deep
anesthesia. In growing pig models, the viability of small bowel graft remained
for 72 h after cold IR in eight of 10 pigs. In experimental group, serum NO
level at 1 and 24 h after reperfusion increased significantly when compared with
control group at the same time point (152.2+/-61.4 micromol/L vs 60.8+/-31.6
micromol/L, t=2.802, P=0.02<0.05; 82.2+/-24.0 micromol/L vs 54.0+/-24.3
micromol/L, t=2.490, P=0.04<0.05). Serum NO level increased significantly at 1 h
post-reperfusion when compared with the same group before cold IR, 24 and 48 h
post-reperfusion (152.2+/-61.4 micromol/L vs 75.6+/-16.2 micromol/L, t=2.820,
P=0.02<0.05, 82.2+/-24.0 micromol/L, t=2.760, P=0.03<0.05, 74.2+/-21.9
micromol/L, t=2.822, P=0.02<0.05). Serum NOS activity at each time point had no
significant difference between two groups. In experimental group, intestinal
mucosal NOS activity at 1 h post-reperfusion reduced significantly when compared
with pre-cold IR (0.79+/-0.04 U/mg vs 0.46+/-0.12 U/mg, t=3.460, P=0.009<0.01).
Mucosal NOS activity at 24, 48, and 72 h post-reperfusion also reduced
significantly when compared with pre-cold IR (0.79+/-0.04 U/mg vs 0.57+/-0.14
U/mg, t=2.380, P=0.04<0.05, 0.61+/-0.11 U/mg, t=2.309, P=0.04<0.05, 0.63+/-0.12
U/mg, t=2.307, P=0.04<0.05). In control group, mucosal NOS activity at 1 and 24
h post-reperfusion was significantly lower than that in pre-cold IR (0.72+/-0.12
U/mg vs 0.60+/-0.07 U/mg, t=2.320, P=0.04<0.05, 0.58+/-0.18 U/mg, t=2.310,
P=0.04<0.05). When compared to the normal value, Na+-K+-ATPase activity
increased significantly at 48 and 72 h post-reperfusion in experimental group
(2.48+/-0.59 micromol/mg vs 3.89+/-1.43 micromol/mg, t=3.202, P=0.04<0.05,
3.96+/-0.86 micromol/mg, t=3.401, P=0.009<0.01) and control group (2.48+/-0.59
micromol/mg vs 3.58+/-0.76 micromol/mg, t=2.489, P=0.04<0.05, 3.67+/-0.81
micromol/mg, t=2.542, P=0.03<0.05). CONCLUSION: This novel technique for
intestinal autotransplantation provides a potentially consistent and practical
model for experimental studies of graft cold preservation. L-arginine
supplementation during cold IR may act as a useful adjunct to preserve the
grafted intestine.
PMID: 15962385 [PubMed - indexed for MEDLINE]
24:
World J Gastroenterol. 2005 Jan 21;11(3):353-6. Effect of NS-398 on colon cancer cells.
Jia XQ, Zhong N, Han LH, Wang JH, Yan M, Meng FL, Zhang SZ.
Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan
250012, Shandong Province, China.
xiaoqingjia@126.comAIM: To study the effect of NS-398, a selective cyclooxygenase-2 (COX-2)
inhibitor, on invasion of colon cancer cell line HT-29 in vitro and to explore
its mechanisms. METHODS: Invasive behaviors of the malignant colon cancer cell
line HT-29 were investigated in this study. Expressions of COX-2 and CD44v6 in
HT-29 cells were detected by flow cytometry. Cellular survival rate was
determined by MTT assay. The invasive capacity was quantified by a modified
Boyden chamber model. Alterations of cytoskeleton component F-actin were
observed by confocal laser scanning microscope. RESULTS: Flow cytometry analysis
showed that COX-2 was highly expressed in HT-29 cells. The invasive capability
of HT-29 cells could be greatly inhibited by NS-398 at the experimental
concentrations of 0.1, 1.0 and 10 micormol/L with an inhibitory rate of 22.74%,
42.35% and 58.61% (P<0.01), respectively. MTT assay showed that NS-398 at the
experimental concentrations had no significant influence on cellular viability,
indicating that such anti-invasive effects had no relationship with
cytotoxicity. F-actin was mainly distributed around nuclei forming annular
structure in HT-29 cells. After exposure to NS-398 of 10 micromol/L, the annular
structure around nuclei disappeared and the fluorescence intensity of F-actin
decreased obviously. Treatment with NS-398 could down-regulate the expression of
CD44v6 as well. CONCLUSION: NS-398 has anti-invasive effects on colon cancer
HT-29 cells in vitro, which may be mediated by a novel mechanism of disruption
of cytoskeleton. Down-regulation of CD44v6 expression may be related to
alterations of cytoskeleton.
PMID: 15637743 [PubMed - indexed for MEDLINE]
