A Ukrainian SCI journal.
Exp Oncol. 2006 Dec;28(4):282-7.
Establishment of serum protein pattern for screening colorectal cancer using
SELDI-TOF-MS.
Zheng GX, Wang CX, Qu X, Deng XM, Deng BP, Zhang J.
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Ji'nan,
Shandong Province, PR China.
AIM: The purpose of this study is to develop a proteomic pattern for
distinguishing individuals with colorectal cancer from healthy controls and
monitoring micrometastasis using SELDI-TOF-MS. METHODS: A training set consisting
of 63 patients with colorectal cancer, 20 patients with benign colorectal
diseases and 26 healthy volunteers was used to develop a proteomic model that
discriminated colorectal cancer effectively. The sensitivity and specificity of
this model was validated by an independent test set. To explore serum proteins
changed after operation, the protein profiles of 31 postoperative patients were
compared with those of preoperative patients. We also analyzed protein profiles
of patients with and without metastasis to monitor micrometastasis. RESULTS: Our
study yielded a four-peak model (m/z: 3191.5, 3262.9, 3396.3 and 5334.4) that
discriminated cancer from non-cancer samples with sensitivity of 90.3% and
specificity of 95.7%. This model was validated in the test set with sensitivity
of 87.5% and specificity of 93.8% which was significantly better than the
combination use of CEA, CA199 and CA242 (sensitivity 62.4%) for early detection
of colorectal cancer. Two peaks (m/z: 2753.8 and 4172.4) were found
down-regulated in postoperative samples comparing with preoperative samples. We
also detected two proteins (m/z: 9184.4 and 9340.9) that can discriminate
patients with primary colorectal cancer from metastatic colorectal cancer.
CONCLUSIONS: The four-peak model and two peaks (m/z: 2753.8 and 4172.4) detected
in this study have the potential for assistance in diagnostics and therapeutic
strategies in colorectal cancer and the two proteins (m/z: 9184.4 and 9340.9)
were effective biomarkers for monitoring micrometastasis.
