CURRICULUM VITAE
Yusen Liu, PhD
Associate Professor of Pediatrics
Center for Developmental Pharmacology and Toxicology
Children’s Research Institute
Department of Pediatrics
The Ohio State University College of Medicine
700 Children’s Drive
Columbus, Ohio 43205
Phone: 614-722-3073
Fax: 614-722-2774
Email:
liuy@pediatrics.ohio-state.eduSEX: Male
DATE AND PLACE OF BIRTH: Oct. 17, 1963
People’s Republic of China
MARITAL STATUS: Married, with two children
NATIONALITY: Chinese
VISA STATUS: U.S. Permanent Resident
PRESENT HOME ADDRESS: 7688 Hidden Hollow Drive
Columbus, OH 43235
Phone: 614-785-1998
PRESENT WORK ADDRESS: Center for Developmental Pharmacology and Toxicology
Children’s Research Institute
Children’s Hospital
Department of Pediatrics
Ohio State University
700 Children’s Drive
Columbus, OH 43205
Phone: 614-722-3073
FAX: 614-722-2007
E-mail:
liuy@pediatrics.ohio-state.eduPRESENT POSITION & TITLE: Principal Investigator
EDUCATION:
Sept., 1979-July, 1983: B. S., Zhejiang University, Hangzhou, China.
Sept., 1983-Sept., 1986: M. S., Dalian Institute of Technology, China.
Nov., 1987-June, 1991: Ph. D., Hiroshima University, Japan.
Major: Molecular Biology and Yeast Genetics.
RESEARCH AND PROFESSIONAL EXPERIENCE:
Sept., 1986-Oct., 1987: Research associate at Department of Chemical
Engineering, Dalian Institute of Technology.
July, 1991-Feb., 1992: Assistant Professor in Department of Fermentation
Technology, Hiroshima University,
Higashi-Hiroshima, Japan.
Mar., 1992-July, 1995: Visiting Fellow, Laboratory of Molecular Genetics,
Gerontology Research Center, National Institute on Aging, NIH,
Baltimore, Maryland.
July, 1995-Nov., 1996: Visiting Associate, Laboratory of Cellular and Molecular
Biology, Gerontology Research Center, National
Institute on Aging, NIH, Baltimore, Maryland.
Dec., 1996-Jan. 2003: Investigator. Laboratory of Cellular and Molecular Biology,
Gerontology Research Center, National Institute on Aging, NIH,
Baltimore, Maryland.
Jan., 2003-present: Principal Investigator, Center for Developmental Pharmacology
and Toxicology, Children’s Research Institute, Children’s
Hospital, 700 Children’s Drive, Columbus, OH 43205
Jan., 2003-present: Assistant Professor of Pediatrics, Department of Pediatrics, The
Ohio State University
Oct., 2003-present: Faculty member, Molecular, Cellular, and Developmental
Biology Graduate Program, The Ohio State University
SUPERVISORY/MANAGERIAL EXPERIENCE:
Supervised 11 postdoctoral fellows (Wei Chen, Dottie Hutter,
Aijung Kim, Lixin Feng, Pinghu Liu, Peili Chen, Ji Li, Natasha
Sinogeeva, Qun Zhao, Lili Liu, and Edward Shepherd), and 3
technicians (Janice Barnes, Trudy Kokkonen, and Marco
Pinero), and 7 summer research students.
Managed a stress signaling research program in NIH. Running
an independent research program in Children’s Research
Institute
PROFESSIONAL SOCIETY: The American Society for Biochemistry and Molecular Biology
since 1995.
The Gerontology Society of America since 1998.
Society for Pediatric Research since 2003
AWARDS AND SCHOLARSHIPS:
Oct., 1987-June, 1991: Japanese Government Scholarship (Scholarship of
Ministry of Education, Culture and Science of Japan).
Mar., 1992-July, 1995: NIH Fogarty Fellowship.
2000 Geron Corporation-Samuel Goldstein Distinguished Publication Award.
INVITED SPEECHES:
1. The Keystone Conference on Signal Transduction in Eukaryotic Cells, Keystone, CO, November
6-9, 1995.
2. 51st Annual Scientific Meeting of The Gerontological Society of America, Philadelphia, PA,
November 20-24, 1998.
3. Annual Scientific Meeting of the American Aging Society, Symposium on Metabolic
Mechanisms of Longevity, Seattle, WA, June 4, 1999.
4. 53st Annual Scientific Meeting of The Gerontological Society of America, Washington D.C.
November 18-22, 2000.
5. Providence College, Providence, RI. Seminar title: Regulation of MAP kinase phosphatases.
November 20, 2001.
6. Lankenau Medical Research Center, Philadelphia, PA. Seminar title: Regulation of nuclear MAP
kinase phosphatases. January 31, 2002.
7. Locus Discovery Inc. Blue Bell, PA. Seminar title: Function and regulation of MAP kinase
phosphatase-1 in macrophages during the cellular response to inflammatory stimuli. June 25,
2002.
8. 2003 Japanese Gerontological Society Annual Meeting, Nagoya, Japan, June 18-20, 2003.
PUBLICATIONS:
1. Liu, Y., Ohki, O., Tachikawa, H., Fukui, S., and Miyakawa, T. (1990) Calmodulin-binding
proteins in Rhodosporidium toruloides-a basidiomycetous yeast. J. Gen. Microbiol. 136, 131-6.
2. Liu, Y., Yamashita, Y., Tsuchiya, E., and Miyakawa, T. (1990) Calmodulin-binding proteins in
Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 166, 681-6.
3. Liu, Y., Ishii, S., Tokai, M., Tsutsumi, H., Ohki, O., Akada, R., Tanaka, K., Tsuchiya, E.,
Fukui, S., and Miyakawa, T. (1991) The Saccharomyces cerevisiae genes (CMP1 and
CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian
protein phosphatase 2B. Mol. Gen. Genet. 227, 52-9.
4. Cui, Z., Mochizuki, D., Matsuno, Y., Nakamura, T., Liu, Y., Hatano, T., Fukui, S., and
Miyakawa, T. (1992) Cloning and molecular analysis of cDNA encoding a
carboxymethylcellulase of the yeast Cryptococcus flavus. Biosci. Biotech. Biochem. 56, 1230-5.
5. Nakamura, T., Liu, Y., Hirata, D., Namba, H., Hirada, S., Hirokawa, T., and Miyakawa, T.
(1993) Protein phosphatase type 2B (calcineurin)-mediated regulation of ion homeostasis is a
determinant of salt tolerance in the yeast Saccharomyces cerevisiae. EMBO J. 12, 4063-71.
(First four authors contributed equally).
6. Liu, Y., Gorospe, M., Yang, C., and Holbrook, N. J. (1995) Role of mitogen-activated protein
kinase phosphatase during the cellular response to genotoxic stress: Inhibition of c-Jun Nterminal
kinase activity and AP-1-dependent gene activation. J. Biol. Chem. 270, 8377-80.
7. Nakamura, T., Namba, H., Ohmoto, T., Liu, Y., Hirata, D., and Miyakawa, T. (1995) Cloning
and characterization of the Saccharomyces cerevisiae SVS1 gene which encodes a serine- and
threonine-rich protein required for vanadate resistance. Gene 165, 25-9.
9. Holbrook, N.J., Liu, Y., and Fornace, Jr., A.J. (1996) Signalling events controlling the molecular
response to genotoxic stress. In Feige U., Morimoto, R.I., Yahara, I., and Polla B.S. (Eds.)
Stress-induced cellular responses. 77, 273-288 (Birkhauser/Springer Press).
10. Liu, Y., Martindale, J., Gorospe, M., and Holbrook, N.J. (1996) Regulation of p21WAF1/CIP1
expression through mitogen-activated protein kinase signalling pathway. Cancer Res. 56, 31-5.
10. Xu, Q., Liu, Y., Gorospe, M., Udelsman, R., and Holbrook, N.J. (1996) Acute hypertension
activates mitogen-activated protein kinases in arterial wall. J. Clin. Invest., 97, 508-14.
11. Gorospe, M., Liu, Y., Chrest, F., and Holbrook, N.J. (1996) Role of G1 checkpoint in the
growth arrest of human breast carcinoma cells by prostaglandin A2. Mol. Cell. Biol., 16, 762-70.
12. Liu, Y., Guyton, K.Z., Gorospe, M., Xu, Q., Kokkonen, T., Mock, Y., Roth, G., and Holbrook,
N.J. (1996) Age-related decline in MAP kinase activity in EGF-stimulated hepatocytes. J. Biol.
Chem., 271, 3604-7.
13. Guyton, K. Z., Liu, Y., Gorospe, M., Xu, Q., and Holbrook, N.J. (1996) Activation of mitogenactivated
protein kinase by H2O2: Role in cell survival following oxidant injury. J. Biol. Chem.,
271, 4138-42.
14. Liu, Y., Guyton, K. Z., Gorospe, M., Xu, Q., Lee, J.C., and Holbrook, N.J. (1996) Differential
activation of ERK, JNK/SAPK and p38/RK/CSBP MAP kinase family members during the
cellular response to arsenite. Free Radical Biol. Med, 21, 771-81.
15. Sheikh, M.S., Garcia, M., Zhan, Q., Liu, Y., and Fornace, A.J., Jr. (1996)
Cell cycle-independent regulation of p21Waf1/Cip1 and retinoblastoma protein during
okadaic acid-induced apoptosisiIs coupled with induction of Bax protein in human
breast carcinoma cells. Cell Growth Differ. 7, 1599-608.
16. Xu, Q., Fawcett, T.W., Gorospe, M., Guyton, K.Z., Liu, Y., and Holbrook, N.J. (1997) Mitogenactivated
protein kinase phosphatase (MKP-1) gene expression in vasculature in response to acute
hypertension. Hypertension 30, 106-11.
17. Huang, Y., Chan, A.M.-L., Liu, Y., Wang, X., and Holbrook, N.J. (1997) Serum
withdrawal and etoposide induce apoptosis in human lung carcinoma cell line A549 via distinct
pathways. Apoptosis 2, 199-206.
18. Liu, Y., Holbrook, N.J., Kokkonen, G.C., and Roth, G.S. (1998) Intracellular signal transduction
and receptor activity in stimulated hepatocyte DNA synthesis. In Yu, B.P. (Eds.) Methods in
Aging Research. 515-32 (CRC Press).
19. Guyton, K.Z., Gorospe, M., Wang, X., Mock, Y.D., Kokkonen, T., Liu, Y., Roth, G., and
Holbrook, N.J. (1998) Age-associated changes in activation of mitogen-activated protein kinase
cascades by oxidative stress. J. Invest. Derm. Symp. Proc., 3, 23-7.
20. Liu, Y., Gorospe, M., Holbrook, N.J., and Anderson, C. (1998) Post-translational mechanisms
leading to mammalian gene activation in response to genotoxic stress. In Hoekstra, M.F. and
Nickoloff, J.A. (Eds.) DNA Damage and Repair, Vol. 2: DNA Repair in Higher Eukaryotes, 263-
298 (Humana Press).
21. Liu, Y., Gorospe, M., Kokkonen, G.C., Boluyt, M.O., Younes, A., Mock, Y.D., Wang, X.,
Roth,G.S., and Holbrook, N.J. (1998) Impairments in both p70 S6 kinase and extracellular
signal-regulated kinase signaling pathways contribute to the decline in proliferative capacity of
aged hepatocytes, Expt. Cell Res. 240, 40-8.
22. Chen, W., Holbrook, N.J., and Liu, Y. (1998) Tumor promoter arsenite activates
mitogen-activated protein kinase through a signaling pathway mediated by epidermal growth
factor receptor and Shc, Mol. Cell Biol. 18, 5178-88.
23. Wang, X., Matindale, J.L., Liu, Y., and Holbrook, N.J. (1998) Mitogen-activated protein (MAP)
kinase signaling pathways and the cellular response to hydrogen peroxide: Differential effects of
ERK, JNK and p38 activities on cell survial, Biochem. J. 333, 291-300.
24. Hutter D, Yo Y, Chen W, Liu P, Holbrook NJ, Roth GS, and Liu Y. (2000) Age-related decline
in Ras/ERK mitogen-activated protein kinase cascade is linked to a reduced association between
Shc and EGF receptor. J Gerontol: A Biol Sci Med Sci. 55, B125-34.
25. Huang, Y., Hutter, D., Liu, Y., Wang, X., Sheikh, M.S., Chan, A.M., and Holbrook, N.J. (2000)
TGF-β1 suppresses serum deprivation-induced death of A549 cells mediated through disparate
effects on c-Jun and JNK activities. Submitted. TGF-β1 suppresses serum deprivation induced
death of A549 cells through differential effects on c-Jun and JNK activities. J. Biol. Chem. 275,
18234-42.
26. Wang, W., Furneaux, H., Cheng, H., Caldwell, M.C., Hutter, D., Liu, Y., Holbrook, N.J., and
Gorospe, M. (2000) HuR regulates p21 mRNA stability by UV light. Mol. Cell. Biol. 20, 760-9.
27. Hutter, D., Chen, P., Barnes, J., and Liu, Y. (2000) Catalytic activation of MAP kinase
phosphatase-1 by binding to p38 MAP kinase: Critical role of the p38 carboxyl-terminal domain
in its negative regulation. Bichem. J., 352 (part 1), 155-63.
28. Shan, S.X., Czar, M.J., Bunnell, S.C., Liu, P., Liu, Y., Schwartzberg, P.L., and Wange, RL.
(2000) Deficiency of PTEN in jurkat T cells causes constitutive localization of Itk/Emt/Tsk to the
plasma membrane and hyper-responsiveness to CD3 stimulation. Mol. Cell. Biol., 20, 6945-57.
29. Liu, Y. and Holbrook, N.J. (2001) Mitogen-activated protein kinase signaling pathways and
aging. In E. J. Masoro & S. Austad (Eds) Handbook of the Biology of Aging. Academic Press.
30. Chen, P., Hutter, D., Yang, X, Gorospe, M., Davis, R.J., and Liu, Y. (2001) Discordance
between the binding affinity of mitogen-activated protein kinase subfamily members for MKP-2
and their ability to catalytically activate the phosphatase. J. Biol. Chem., 276, 29440-49.
31. Tong, T. Fan, W., Zhao, H., Jin, S., Fan, F., Blanck, P., Alomo, I., Rajasekaran, B., Liu, Y.,
Holbrook, N.J., and Zhan, Q. (2001) Involvement of the MAP kinase pathways in induction of
GADD45 following UV radiation. Expt. Cell Res., 269, 64-72.
32. Li, J., Gorospe, M., Hutter, D., Barnes, J., Keyse, S.M., and Liu, Y. (2001) Transcriptional
induction of MKP-1 in response to stress is associated with histone H3
phosphorylation/acetylation. Mol. Cell. Biol., 21, 8213-22.
33. Rong, R., Sheikh M.S., He, Q., Liu, Y., Aaronson, S.A., and Huang, Y. (2002) TC21 induces
transformation and promotes cell survival via activation of phosphatidylinositol 3-kinase/Akt and
NFκ-B signaling pathways. Oncogene, 21, 1062-70.
34. Chen, P., Hutter, D., Liu, P., and Liu, Y. (2002) A mammalian expression system for rapid
production and purification of active MAP kinase phosphatases. Protein Expr. Purif., 24, 481-8.
35. Hutter, D., Li, J., Chen, P., Barnes, J., and Liu, Y. (2002) The carboxyl-terminal domains of
MKP-1 and MKP-2 have inhibitory effects on their phosphatase activity. Mol. Cell. Biochem.,
233, 107-17.
36. Li, J., Chen, P., Sinogeeva, N., Barnes, J., Wersto, R.P., Chrest, F.J., Gorospe, M., and Liu, Y.
(2002) Arsenic trioxide promotes histone H3 modification at the chromatin of CASPASE-10
during apoptosis of acute promyelocytic leukemia cells. J. Biol. Chem., 277, 49504-10.
37. Chen, P., Li, J., Barnes, J., Kokkonen, G.C., Lee, J.C., and Liu, Y. (2002) Restraint of proinflammatory
cytokine biosynthesis by MKP-1 in lipopolysaccharide-stimulated macrophages. J.
Immunol., 69, 6408-16.
38. Li, J., Barnes, J., Gorospe, M., and Liu, Y. (2003) Tumor promoter arsenite stimulates histone
H3 phosphorylation/acetylation at the chromatins of c-fos and c-jun in normal human diploid
fibroblasts. J. Biol. Chem. 278,13183-91.
39. Shepherd, E.G., Zhao, Q., Welty, S.E., Hansen, T.N., Smith, C.V., and Liu, Y. Modulation of
inflammatory signals by mitogen-activated protein kinase phosphatase-1 in macrophages
stimulated with peptidoglycan. Submitted.
RECENT ABSTRACTS:
Chen, P., Hutter, D., Yang, X, Gorospe, M., Davis, R.J., and Liu, Y. (2002) Discordance between the
binding affinity of mitogen-activated protein kinase subfamily members for MKP-2 and their ability to
catalytically activate the phosphatase. Annual Conference of American Society of Biochemistry and
Molecular Biology. April 21-24. New Orleans, Louisiana.
Li, J., Gorospe, M., Hutter, D., Barnes, J., Keyse, S.M., and Liu, Y. (2002) Transcriptional induction of
MKP-1 in response to stress is associated with histone H3 phosphorylation/acetylation. Annual
Conference of American Society of Biochemistry and Molecular Biology. April 21-24. New Orleans,
Louisiana.
Liu, Y., Miller, B.A., and Barnes, B. Interferon- augments p38 and JNK MAP kinases through blocking
MKP-1 induction in lipopolysaccharide-stimulated macrophages. 2003 American Pediatric
Society/Society for Pediatric Research Annual Meeting, May 2-6. Seattle, Washington.
Liu, Y., Li, J., Lorenzini, A., Sinogeeva, N., Chen, P., Gorospe, M., and Cristofalo, V.J. Constitutively
active Raf induces growth arrest in WI-38 normal human lung fibroblasts: The effects of active Raf on
chromosomal structure and gene expression profiles. 2003 American Pediatric Society/Society for
Pediatric Research Annual Meeting, May 2-6. Seattle, Washington.
Chen, P., Li, J., Barnes, J., Kokkonen, G.C., Lee, J.C., and Liu, Y. (2003) Restraint of pro-inflammatory
cytokine biosynthesis by MKP-1 in lipopolysaccharide-stimulated macrophages. 6th International
Congress for Inflammation. August 3-6, Vancouver, Canada.
Manson, M.E., Rogers, L.K., Smith, C.V., and Liu, Y. Overdose of acetaminophen activates c-Jun Nterminal
kinases. 2003 Annual Meeting of Society of Toxicology Ohio Valley chapter. Nov 7,
Cincinnati, Ohio.
Liu, Y., Li, J., Gorospe, M., and Barnes, J. Tumor promoter arsenite stimulates histone H3
phosphoacetylation at c-fos and c-jun in normal human diploid fibroblasts. 2004 Society of Toxicology
Meeting, March 21-25, Baltimore, Maryland.
Zhao, Q., Shepherd, E.G., and Liu, Y. The role of MKP-1 as a negative regulator of map kinases in LPSstimulated
alveolar macrophages. 2004 American Pediatric Society/Society for Pediatric Research Annual
Meeting, May 1-4. San Francisco, California.
Zhao, Q., Shepherd, E.G., and Liu, Y. Induction of mitogen-activated protein kinase phosphatase-1 by
anti-inflammatory corticosteroids in macrophages. 2004 American Pediatric Society/Society for Pediatric
Research Annual Meeting, May 1-4. San Francisco, California.
Shepherd, E.G., Zhao, Q., Welty, S.E., Hansen, T.N., Smith, C.V. and Liu, Y. Modulation of
inflammatory signals by MAP kinase phosphatase-1 in peptidoglycan-stimulated macrophages. 2004
American Pediatric Society/Society for Pediatric Research Annual Meeting, May 1-4. San Francisco,
California.
Liu, L., Su, S.Y., Nowicki, P.N., Wang, X.T., and Liu, Y. Triptolide inhibits the PI3-kinase/Akt pathway
and induces apoptosis of human tumor cells. 2004 American Pediatric Society/Society for Pediatric
Research Annual Meeting, May 1-4. San Francisco, California.
Nelin, L.D., Young, T.L., Smith, C.V., and Liu, Y. The effect of stable transfection of MKP-1 on LPSinduced
nitric oxide and urea production in RAW264.7 cells. 2004 American Pediatric Society/Society
for Pediatric Research Annual Meeting, May 1-4. San Francisco, California.
Young, T.L., Chicoine , L.G., Liu, Y., and Nelin, L.D. Genistein inhibits cytokine-induced arginase
expression and urea production in pulmonary arterial endothelial cells. 2004 American Pediatric
Society/Society for Pediatric Research Annual Meeting, May 1-4. San Francisco, California.
Meng, X., Manson. M.E., Rogers, L.K., Shepherd, E.G., Smith, C.V., and Liu, Y. Overdose of
acetaminophen activates c-Jun N-terminal kinases. 2004 American Pediatric Society/Society for Pediatric
Research Annual Meeting, May 1-4. San Francisco, California.
Liu, Y., Zhao, Q., Shepherd, E.G., Chen, P., and Kokkonen, GC. (2004) Regulation of proinflammatory
cytokine production by mitogen-activated protein kinase phosphatase-1 in lipopolysaccharide-stimulated
macrophages. ASMBM annual meeting and 8th IUBMB June 12-16, Boston, Massachusetts.
FUNDING:
PAST
1996-2003 Direct funding from the NIH/NIA-Intramural Research Program
Approximately $400,000 per year
ACTIVE
1 R43 HL 076053-01 (Production of Human CD39 as an Antithrombotic Agent)
Soonseog Jeong, Pricipal Investigator
Yusen Liu, Consultant at 5%
NIH/NHLBI, 01/16/2004 – 07/16/2004, Direct Cost: $79,000
1 R21 AI57798-01 (MKP-1 in Regulation of Inflammatory Cytokine Production)
Yusen Liu, Principal Investigator at 20%
NIH/NIAID, 3/15/2004 – 3/14/2005, Direct Cost: $225,000
PENDING
1 R01 AI57798-01A1 (MKP-1 in Regulation of Inflammatory Cytokine Production)
Yusen Liu, Principal Investigator at 25%
NIH/NIAID, 07/01/2004 – 06/30/2009, Annual Direct Cost Requested: $225,000
Priority Score : 160; Percentile: 11.6%
1 R01 AG025179-01 (Age-related Changes in Hepatocyte Signaling Mechanisms)
Yusen Liu, Principal Investigator at 30%
NIH/NIA, 11/01/2004 – 10/31/2009, Annual Direct Cost Requested: $250,000
POSTDOCTORAL TRAINEES:
NAME: SUPPORT DATE
Wei Chen NIH-Intramural Research Program 6/1997-6/1998
Lixin Feng NIH-Intramural Research Program 1/1998-2/1999
Aijung Kim NIH-Intramural Research Program 6/1998-12/1998
Dorothy E. Hutter NIH-Intramural Research Program 6/1998-8/2000
Pinghu Liu NIH-Intramural Research Program 9/1998-11/2000
Peili Chen NIH-Intramural Research Program 4/1999-12/2001
Ji Li NIH-Intramural Research Program 6/2000-3/2002
Natasha Sinogeeva NIH-Intramural Research Program 5/2001-6/2002
Edward G. Shepherd NIH Training Grant 4/2003-present
Lili Liu Children’s Internal Research Fund 6/2003-1/2004
Qun Zhao Children’s Internal Research Fund 5/2003-present
JOURNAL REVIEW:
Blood, Oncogene, Experimental Cell Research, Cancer Research, Protein Expression and Purification,
Biochimica Biophysica Acta, Journal of Gerontology, Circulation Research, Molecular Biology of the
Cell.
GRANT REVIEW:
The Wellcome Trust (Mail Reviewer), 2003
http://www.ibgp.org/faculty/profilepage.asp?ID=390
