引用第5楼liu_redsnow于2007-01-02 17:41发表的“”:比之齐鲁逊一筹统计是省立的文章多是CHEN ZJ相关其他人的少
子江。
1: Eur J Obstet Gynecol Reprod Biol. 2006 Dec;129(2):150-4. Epub 2006 May 4.
Influence of swim-up time on the ratio of X- and Y-bearing spermatozoa.
Yan J, Feng HL, Chen ZJ, Hu J, Gao X, Qin Y.
Center for Human Reproduction, Shandong University and Shandong Provincial
Hospital, Jingwu Weiqi Road No. 324, Jinan, China.
OBJECTIVE: The objective was to examine the separation of X- and Y-bearing
spermatozoa in a modified swim-up procedure using fluorescent in situ
hybridization (FISH), and to find out the influence of swim-up time on the ratio
of X- and Y-bearing spermatozoa. STUDY DESIGN: Prospective study. SETTING:
Reproductive testing laboratory in a university hospital. PATIENTS: Normal
spermatozoa samples were obtained from 10 volunteers by masturbation after
sexual abstinence for 3-5 days. INTERVENTIONS: Spermatozoa were put into 18
tubes with 0.25ml in each, then mixed with HTF medium and centrifuged for 5min
(400xg). The supernatant was removed and discarded and 0.5ml HTF was added
slowly along the tube wall. Motile spermatozoa were collected after swimming up
in different times (from 5 up to 150min, with a total of 17 intervals). The X-
and Y-bearing spermatozoa were determined using the FISH technique. The X/Y
dual-color CEP probes that were marked by fluorescein isothiocyanate (FITC) and
Texas red were applied to analyze the ratio of X- and Y-bearing spermatozoa. The
FISH staining slides were analyzed under an immunofluorescence microscope. About
1000-1500 spermatozoa were counted per slide. MAIN OUTCOME MEASURES: The
percentages of X- and Y-bearing spermatozoa were calculated. RESULTS: The study
results suggested that the total ratio of hybridization was 98.33%. The ratio of
X-bearing spermatozoa after swimming up for different amounts of time is
50.03+/-0.91% at 0min, 50.45+/-2.06% after 15min, 50.61+/-2.47% after 30min,
50.16+/-2.67% after 60min, 50.72+/-2.64% after 90min, and 50.56+/-2.20% after
150min. The statistical analysis showed that there were no significant
differences among different swim-up times in the ratio of X-bearing spermatozoa.
CONCLUSIONS: There was no significant effect of swim-up time on the ratios of X-
and Y-bearing spermatozoa using a modified swim-up procedure. No direct evidence
was found that the swim-up procedure for separating motile spermatozoa to use
for either intrauterine insemination (IUI) or in vitro fertilization (IVF) would
lead to an imbalance of boys and girls.
PMID: 16678330 [PubMed - in process]
2: Fertil Steril. 2006 Apr;85(4):827-32.
Comment in:
Fertil Steril. 2006 Apr;85(4):833-5; discussion 841.
Fertil Steril. 2006 Apr;85(4):836-7; discussion 841.
Fertil Steril. 2006 Apr;85(4):838; discussion 841.
Fertil Steril. 2006 Apr;85(4):839-40; discussion 841.
Confocal microscopic analysis of the spindle and chromosome configurations of
human oocytes matured in vitro.
Li Y, Feng HL, Cao YJ, Zheng GJ, Yang Y, Mullen S, Critser JK, Chen ZJ.
Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong
University, Jinan, China.
OBJECTIVE: To assess the potential effects of in vitro maturation (IVM) of human
oocytes on the meiotic spindle and associated chromosome configuration. DESIGN:
Prospective study. SETTING: Hospital-based IVF center. PATIENT(S): Patients with
polycystic ovary syndrome (PCOS) undergoing unstimulated and stimulated cycles
of oocyte retrieval. INTERVENTION(S): Immature (germinal vesicle and metaphase
I) and mature (metaphase II) oocytes were collected from PCOS patients. The
meiotic spindle and chromosome configurations in oocytes matured in vitro and in
vivo were studied by confocal microscopy, with fluorescent labeling techniques
for visualization of both microtubules and chromatin. MAIN OUTCOME MEASURE(S):
Meiotic spindle and associated chromosome configurations. RESULT(S): Oocytes can
develop to the metaphase II stage after IVM. Confocal microscopic observations
revealed that the oocytes matured in vitro had a higher frequency of abnormal
meiotic spindle and chromosomal alignment morphology than in vivo-matured
oocytes. These abnormalities included a partial or total disorganization of the
meiotic spindle microtubules. Abnormal chromosome organization included
dispersal of chromosomes or chromosomes with an aberrant, less-condensed
appearance. The proportions of abnormality in spindle and chromosome
configurations in oocytes matured in vitro were 43.7% and 33.3%, respectively,
which was significantly higher than in those oocytes matured in vivo (13.6% and
9.1%). CONCLUSION(S): In vitro maturation can have deleterious effects on the
organization of the meiotic spindle and chromosome alignment of human oocytes.
This result suggests one possible explanation for the reduced developmental
potential of oocytes matured in vitro compared with those matured in vivo. This
is likely a contributing factor to the overall lower clinical outcomes observed
after IVM and ET.
Publication Types:
Comparative Study
Research Support, Non-U.S. Gov't
PMID: 16580356 [PubMed - indexed for MEDLINE]
3: Eur J Obstet Gynecol Reprod Biol. 2006 May 1;126(1):72-6. Epub 2005 Dec 13.
Combination of calcium ionophore A23187 with puromycin salvages human
unfertilized oocytes after ICSI.
Lu Q, Zhao Y, Gao X, Li Y, Ma S, Mullen S, Critser JK, Chen ZJ.
Reproductive Medical Center, Shandong Provincial Hospital, Shandong University,
Jinan 250021, China.
OBJECTIVE: To determine whether oocyte activation using a combination of calcium
ionophore A23187 (A23187) with puromycin could salvage human unfertilized
oocytes after ICSI. STUDY DESIGN: One hundred and thirteen discarded
unfertilized oocytes 20-68 h after ICSI were assigned to four groups: ICSI 20-h
group, ICSI 44-h group, ICSI 68-h group and control. All unfertilized oocytes
were exposed to A23187 (5 microM) for 5 min and subsequently were incubated with
puromycin (10 microg/ml) for 4 h. Sex chromosomal analysis was performed by dual
color fluorescence in situ hybridization (FISH). RESULTS: The combination of
A23187 with puromycin could activate the unfertilized oocytes 20-68 h after
ICSI. The best results were achieved in the ICSI 20-h group, which exhibited an
activation rate of 91.2% (31/34), a cleavage rate of 64.7% (22/34) and 44.1%
(15/34) high-quality embryos. The activation rate, cleavage rate and the number
of high-quality embryos appeared to decrease with the cultured time of
unfertilized oocytes after ICSI. FISH analysis showed six embryos with XX and
seven embryos with XY in 16 embryos derived from 2PN2PB. CONCLUSIONS: The
combination of calcium ionophore A23187 with puromycin could effectively salvage
unfertilized oocytes within 20 h after ICSI.
Publication Types:
Research Support, Non-U.S. Gov't
PMID: 16352389 [PubMed - indexed for MEDLINE]
4: Int J Gynaecol Obstet. 2004 Sep;86(3):394-5.
Rupture of pregnancy in a communicating rudimentary uterine horn after in vitro
fertilization and embryo transfer.
Tang R, Sheng Y, Chen ZJ.
Department of Ob and Gyn, Reproductive Research Center, Shandong Provincial
Hospital, Shandong University, Jinan 250021, China.
Publication Types:
Case Reports
PMID: 15325862 [PubMed - indexed for MEDLINE]
5: Hum Reprod. 2004 Oct;19(10):2345-9. Epub 2004 Aug 6.
Effects of sucrose concentration on the developmental potential of human
frozen-thawed oocytes at different stages of maturity.
Chen ZJ, Li M, Li Y, Zhao LX, Tang R, Sheng Y, Gao X, Chang CH, Feng HL.
Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong
University, Jinan 250021, China.
BACKGROUND: Success of human oocyte cryopreservation depends on multiple
cryobiological factors that could influence the developmental potential of the
oocytes. The objective of this study was to examine the effects of different
sucrose concentrations on the developmental potential of human frozen-thawed
oocytes at different maturity stages. METHODS: A total of 355 oocytes collected
from small follicles were randomly divided into three groups and two groups (B
and C) were cryopreserved using slow-freezing method. Group A included 131
oocytes at different maturity stages without freezing. Another 119 oocytes in
Group B were cryopreserved with 0.1 M sucrose and 105 oocytes in Group C with
0.2 M sucrose concentration. RESULTS: The post-thaw survival rate of the oocytes
and the cleavage rate in Group C were significantly higher than that of Group B
(P<0.05). For immature metaphase I (MI) stage oocytes, a significant difference
was found in the maturation rate between Group C and Group B (P<0.05). The
maturation rate for the GV oocytes in Groups A and C was significantly higher
than Group B (P<0.01). CONCLUSIONS: The results suggested that sucrose
concentration of 0.2 M in the cryoprotectant solution is more suitable for human
oocyte cryopreservation.
Publication Types:
Research Support, Non-U.S. Gov't
PMID: 15298975 [PubMed - indexed for MEDLINE]
6: J Pharm Pharmacol. 2003 Mar;55(3):353-8.
Quercetin, a phytoestrogen and dietary flavonoid, activates different
membrane-bound guanylate cyclase isoforms in LLC-PK1 and PC12 cells.
Chen ZJ, Vetter M, Chang GD, Liu S, Chang CH.
Department of Medicine, Reproductive Research Center, Shandong Provincial
Hospital, Shandong University, Jinan, P. R. China.
Accumulated evidence suggests that quercetin, a dietary flavonoid, has
beneficial effects in protection against cardiovascular diseases and in the
inhibition of tumour growth. We have recently shown that antioxidants such as
17beta-estradiol, resveratrol, dithiothreitol and vitamin C activate
membrane-bound guanylate cyclase GC-A, a receptor for atrial natriuretic factor
(ANF). Since quercetin is a phytoestrogen and potent antioxidant, it is possible
that it may activate GC-A or other guanylate cyclase isoforms. We examined
whether quercetin activates GC-A or GC-B (the receptor for C-type natriuretic
peptide, CNP) in PC12 and porcine kidney proximal tubular LLC-PK1 cells. The
results showed that quercetin activated a guanylate cyclase isoform in both cell
types. Quercetin inhibited CNP-stimulated GC-B activity, but had little effect
on ANF-stimulated GC-A activity in PC12 cells, suggesting that quercetin mainly
activates GC-B in PC12 cells. In contrast, CNP had no effect on guanylate
cyclase activity in LLC-PK1 cells, indicating that GC-B is not expressed in
LLC-PK1 cells. Furthermore, quercetin had a small effect on ANF-stimulated GC-A
activity and had no effect on soluble guanylate cyclase activity in LLC-PK1
cells, suggesting that quercetin does not activate GC-A, GC-B or soluble
guanylate cyclase in LLC-PK1 cells. However, quercetin did stimulate
membrane-bound guanylate cyclase activity in LLC-PK1 cell membranes. These
results indicate that quercetin activates the GC-B isoform in PC12 cells, but
activates an unknown membrane-bound guanylate cyclase isoform in LLC-PK1 cells.
Publication Types:
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
PMID: 12724041 [PubMed - indexed for MEDLINE]
7: J Pharm Pharmacol. 2001 Feb;53(2):243-7.
Antioxidants, vitamin C and dithiothreitol, activate membrane-bound guanylate
cyclase in PC12 cells.
Chen ZJ, Che D, Chang CH.
Department of Medicine, Reproductive Research Center, Shandong Provincial
Hospital, Shandong Medical University, Jinan, Peoples Republic of China.
Antioxidants and antioxidant enzymes are known to protect against cell death
induced by reactive oxygen species. However, apart from directly quenching free
radicals, little is known about the effect of antioxidants on hormone-activated
second messenger systems. We previously found that antioxidants such as 17-beta
estradiol and resveratrol activate membrane-bound guanylate cyclase GC-A, the
receptor for atrial natriuretic factor (ANF), in PC12 cells. It is possible that
other antioxidants may also activate membrane-bound guanylate cyclase GC-A. The
aim of this study was to determine if dithiothreitol (DTT), vitamin C, and
vitamin E activate membrane-bound guanylate cyclase GC-A in PC12 cells. The
results showed that both DTT and vitamin C increased cGMP levels in PC12 cells,
whereas vitamin E had no effect. DTT and vitamin C inhibited membrane-bound
guanylate cyclase activity stimulated by ANF in PC12 cells. In contrast, DTT and
vitamin C had no effect on soluble guanylate cyclase activity stimulated by
substance P. Furthermore, NO synthase inhibitors L-NAME and aminoguanidine did
not affect DTT- and vitamin C-stimulated guanylate cyclase activity. The results
indicate that DTT and vitamin C, but not vitamin E, activate membrane-bound
guanylate cyclase GC-A in PC12 cells.
Publication Types:
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
PMID: 11273022 [PubMed - indexed for MEDLINE]
8: Chin Med J (Engl). 1992 Sep;105(9):785-7.
Delivery by direct intrauterine transfer of gametes.
Su YK, Chen ZJ, Feng Y, Liu LK, Li JJ, Liu XM.
Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Jinan.
A nurse, 27, primary sterility for 5 years, had normal menstruation, normal size
of uterus anterior. HSG: Rt. tube was not visible, Lt. tube slightly enlarged at
ampulla. Ovaries were over-stimulated by clomiphene, HMG and hCG.8 ova were
taken through vaginal aspiration under B-mode ultrasound scanning. Direct
introduction of ova and washed sperms simultaneously into the uterus on Sept.
19, 1991 resulted in successful single pregnancy up to 36(+1) weeks. A 2 250 g
normal baby boy was delivered on May 15, 1992 spontaneously. The base and
prospect of this manoeuver are discussed with a reference to Craft's procedure.
PMID: 1288984 [PubMed - indexed for MEDLINE]
