Cytoskeleton: Signalling and Cell Regulation: a Practical Approach
Practical Approach Series ; 221
by Carraway, K. L. Carraway, C. A. C.
From Inside the eBook
Giovanna Bermano and John E. Hesketh describe how widely-available techniques can be used to address such questions and so assist the reader in determining the extent and mechanism of mRNA-cytoskeleton interactions. Thus, recovery of polysomes or a specific mRNA in the cell matrix does not necessarily mean that they are associated with the cytoskeleton. (Page 212)
10: The study of mRNA-cytoskeleton interactions anchoring of mRNAs (microfilaments) Figure 2. mRNA sorting and the cytoskeleton. Meanwhile, with present techniques of biochemical fractionation and in situ hybridization, it is possible to address whether a specific mRNA of interest is associated with the cytoskeleton, whether it is localized, whether the targeting of the mRNA involves the 3' UTR, and which proteins interact with the mRNA and the cytoskeleton. (Page 211)
Rinse coverslips briefly with two exchanges of cytoskeleton buffer and fix for an additional 10 min in fixative B. 3. Following fixation, rinse coverslips in cytoskeleton buffer for 5 min, and PBS for an additional 10 min. 4. (Page 117)
