【题名】： Preparation and determination of desethylamiodarone in dog lung by HPLC-MS
【作者】：Authors: Hong, Liu Xue; Ying, Yang Chuan; Lei, Zheng; Chen, Guo Rui
【杂志名全称】：pharmazie
【年, 卷(期), 起止页码】：Volume 66, Number 11, November 2011 , pp. 822-825(4)
【DOI】：DOI:
http://dx.doi.org/10.1691/ph.2011.1071【全文链接】：
http://www.ingentaconnect.com/content/govi/pharmaz/2011/00000066/00000011/art00002【摘要信息】：A novel method was developed for the preparation and determination of desethylamiodarone in dog lung by high-performance liquid chromatography (HPLC) with amiodarone as a standard. The selected dog was orally given amiodarone, then executed and the active metabolite desethylamiodirone in lung tissue was isolated, concentrated and purified by Waters C18 column (25 mm × 250 mm,10 m) with mobile phase of acetonitrile-100mmol/L acetic acid containing 15 mmol/L diethylamine (55:45 v/v) at a flow rate of 10.0 mL/min. Hypersil ODS2 column (4.6 mm × 250 mm 5 m) was used to analyze amiodarone and desethylamiodarone, with the same mobile phase at a flow rate of 1.0 mL/min, the detection wavelength was 237.5 nm. Atmosperic pressure electronic spray ionization (AP-ESI) and ion mass spectral (m/z) of 618.1(M + H) were selected to validate desethylamiodarone. The fi of desethylamiodarone and amiodarone were 1.04 ± 0.02 and 1.020 ± 0.01, respectively. It indicated that desethylamiodarone can be separated and purified by preparational HPLC after Mass Spectrometry (MS) validation and quantified according to fi of amiodarone indirectly. The proposed method enables the preparation and determination of desethylamiodarone in dog lung successfully.
【求助者email】：
zhenglei8501@163.com[ 此帖被zhenglei8501在2011-11-25 13:54重新编辑 ]
